Overexpression of CfPIP1 - 1 , CfPIP1 - 2 , and CfPIP1 - 4 genes of Catalpa fargesii in transgenic Arabidopsis thaliana
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ORIGINAL PAPER
Overexpression of CfPIP1‑1, CfPIP1‑2, and CfPIP1‑4 genes of Catalpa fargesii in transgenic Arabidopsis thaliana under drought stress Wenjun Ma1 · Yao Xiao1 · Yuan Li1,3 · Pan Hu1,2 · Zhi Wang1 · Guijuan Yang1 · Junhui Wang1
Received: 14 August 2019 / Accepted: 9 October 2019 © Northeast Forestry University 2020
Abstract Catalpa fargesii is an important economic tree species used for furniture and timber production because of its high density and hardness. Its survival and growth are severely affected and primarily limited by drought stress. Thus, to better understand the mechanism of drought resistance in C. fargesii, we used qRT-PCR to reveal significantly different expression of three plasma membrane intrinsic protein genes: CfPIP1-1, CfPIP1-2 and CfPIP1-4. We then cloned their full-length cDNA sequences and characterized the encoded proteins. We analyzed the genes phylogenetically and predicted conserved motifs, domains, and secondary and tertiary structures. To verify the function of Project funding: The work was supported by Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2014QA004). The online version is available at http://www.springerlink.com Corresponding editor: Tao Xu. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11676-019-01082-w) contains supplementary material, which is available to authorized users. * Junhui Wang [email protected] 1
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, National Forest Genetic Resource Platform, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, People’s Republic of China
2
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People’s Republic of China
3
Central South University of Forestry and Technology, Changsha 410004, People’s Republic of China
the CfPIP1 genes further, we ectopically expressed CfPIP1 transgenes in Arabidopsis thaliana. The results showed that CfPIP1-1, CfPIP1-2 and CfPIP1-4 had several characteristics of aquaporins. The transgenic plants grew better than the WT plants did under drought stress, and overexpression of the CfPIP1 genes increased the plant water content and resistance to drought. Thus, CfPIP1-1, CfPIP1-2 and CfPIP1-4 of C. fargesii play key roles in regulating the intracellular and extracellular water balance and in mediating the plant response to drought. Keywords Catalpa fargesii · Drought tolerance · PIP1 · Aquaporin · Heterologous transformation
Introduction Water is one of the most important factors for plant growth and development. Drought promotes changes in forest structure and reduces tree growth in forests (Bunker and Carson 2005; Dulamsuren et al. 2010). Efforts to protect natural forest resources have increased, and plantation areas have increased worldwide (Payn et al. 2015). To meet the demand for commercial timber, some plantations are also established
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