Partially saturated canthaxanthin alleviates aging-associated oxidative stress in d -galactose administered male wistar
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RESEARCH ARTICLE
Partially saturated canthaxanthin alleviates agingassociated oxidative stress in D-galactose administered male wistar rats Ahila Mathimaran . Anbarasu Kumar . Gurudayal Prajapati . Ravi S. Ampapathi . Himangsu K. Bora . Rajdeep Guha
Received: 7 July 2020 / Accepted: 5 September 2020 Ó Springer Nature B.V. 2020
Abstract It has been earlier reported that partially saturated canthaxanthin (PSC) from Aspergillus carbonarius mutant is non-toxic, has anti-lipid peroxidation activity and can induce apoptosis in prostate cancer cell lines. In the present study, the antiaging effect of PSC was explored in D-galactose administered male wistar rats. 8–10 weeks old, male wistar rats were randomly divided into (i) Vehicle Control Group (VCG), (ii) Aged Control Group (ACG), (iii) Aged ? a Lipoic Acid Group (ALG) and (iv)
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10522-020-09898-4) contains supplementary material, which is available to authorized users. A. Mathimaran A. Kumar (&) Department of Biotechnology, Periyar Maniammai Institute of Science & Technology, Vallam, Thanjavur, Tamil Nadu, India e-mail: [email protected] A. Mathimaran H. K. Bora R. Guha Laboratory Animal Facility, CSIR-Central Drug Research Institute (CSIR-CDRI), Lucknow, Uttar Pradesh, India G. Prajapati R. S. Ampapathi Sophisticated Analytical Instrument Facility, CSIRCentral Drug Research Institute (CSIR-CDRI), Lucknow, Uttar Pradesh, India
Aged ? Partially saturated canthaxanthin Group (APG). Rats received D-galactose (300 mg /kg bwt/day; i.p.) alone (ACG) or together with PSC (APG) (20 mg/kg bwt/day; oral) and a Lipoic Acid (ALG) (80 mg/kg bwt/day; oral) for 10 weeks. Rats in VCG were injected with the same volume of physiological saline (i.p.) and fed with olive oil (vehicle). In vitro protein oxidation and DNA oxidation inhibition, in vivo malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), acetylcholinesterase (AChE) and monoamine oxidase (MAO) activities were determined. In addition, brain neurotransmitters, dopamine and serotonin were estimated by NMR. PSC treatment showed inhibition against protein and DNA oxidation. PSC effectively improved D-galactose induced aging rats by inducing a protective effect through upregulation of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and brain neurotransmitters and downregulated malondialdehyde (MDA) and monoamineoxidase (MAO) levels. Thus, PSC appears to be a functional compound having antioxidant and antiaging properties. Keywords Aging Oxidative stress Carotenoids Antiaging Antioxidant
R. S. Ampapathi R. Guha (&) CSIR-Academy of Scientific and Innovative Research (CSIR-AcSIR), Ghaziabad, Uttar Pradesh, India e-mail: [email protected]
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Biogerontology
Introduction Aging is one of the complex and multifactorial biological process which affects the central nervous system and immune sys
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