Patients with non-relapsing ANCA-associated vasculitis have increased numbers of circulating IL-10 producing Th17 cells
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POSTER PRESENTATION
Open Access
Patients with non-relapsing ANCA-associated vasculitis have increased numbers of circulating IL-10 producing Th17 cells B Wilde1,2, M Thewissen1, P van Paassen1, M Hilhorst1, J Damoiseaux1, O Witzke2, J W Cohen Tervaert1* From 5th European Workshop on Immune-Mediated Inflammatory Diseases Sitges-Barcelona, Spain. 1-3 December 2010 Introduction/aim IL-17 producing T-cells (Th17) were recently defined as a new, pro-inflammatory T-cell subset and are considered to have a key role in autoimmune diseases. Importantly, it was recently described that anti-inflammatory regulatory T-cells (Treg) are able to convert to pro-inflammatory Th17 cells (“Plasticity”) and vice versa. Little is known about the Th17 response or plasticity in ANCA-associated vasculitis (AAV). Therefore, we investigated Th17 responses in AAV. Patients and methods 47 patients with ANCA-associated vasculitis and 12 agematched healthy controls (HC) were studied. PBMC were isolated by ficoll gradient centrifugation and stimulated for 4 hours with phorbol-myristate-acetate/ionomycin in presence of brefeldin A. Intracellular staining was performed to detect IFNg, IL-4, IL-10 and IL-17A producing T-helper-cells by flow cytometry. Ten renal biopsies with necrotizing-crescentic-glomerulonephritis (NCGN) were stained for IL-17 by immunohistochemistry. Results AAV patients in remission (n=27) and with active disease (n=20) had increased numbers of circulating IL-17A + T-helper-cells as compared to HC (2.04 ±1.65% vs. 0.73 ±0,36%, p
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