Sophorolipid Production Using Lignocellulosic Biomass by Co-culture of Several Recombinant Strains of Starmerella bombic
- PDF / 789,816 Bytes
- 12 Pages / 439.37 x 666.142 pts Page_size
- 31 Downloads / 157 Views
Sophorolipid Production Using Lignocellulosic Biomass by Co-culture of Several Recombinant Strains of Starmerella bombicola with Different Heterologous Cellulase Genes from Penicillum oxalicum Yue Li 1 & Na Gao 1 & Xinyu Zhang 1 & Guoqin Zhao 1 & Xin Song 1,2 Received: 24 August 2020 / Accepted: 28 September 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
One of the reasons hindering large-scale application of sophorolipids (SLs) is high production cost. In this study, six recombinant strains of Starmerella bombicola, sbEG1, sbEG2, sbCBH1, sbCBH1–2, sbBGL1, and sbCBH2 expressing cellulase genes eg1, eg2, cbh, cbh1–2, bgl1, and cbh2 from Penicillium oxalicum were respectively constructed. Four strains showed cellulase activities and were co-cultivated in fermentation media containing 2% glucose, 1% Regenerated Amorphous Cellulose (RAC), 2% glucose, and 1% RAC, respectively. After 7 days’ cultivation, concentration of SLs in medium with 1% RAC (g/L) reached 1.879 g/L. When 2% glucose and 1% of RAC were both contained, the titer of SLs increased by 39.5% than that of control strain and increased by 68.8% than that in the medium with only 2% glucose. Results demonstrated that cellulase genes from filamentous fungi in S. bombicola can function to degrade lignocellulosic cellulose to produce SLs. Keywords Starmerella bombicola . Sophorolipids . Cellulase gene . Lignocellulosic biomass . Co-culture
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12010-02003433-4) contains supplementary material, which is available to authorized users.
* Xin Song [email protected]
1
State Key Laboratory of Microbial Technology, Shandong University, Binhai Road 72, Qingdao 266237 Shandong, People’s Republic of China
2
National Glycoengineering Research Center, Shandong University, Binhai Road 72, Qingdao 266237 Shandong, People’s Republic of China
Applied Biochemistry and Biotechnology
Introduction Biosurfactants produced through microbial fermentation have attracted increasing attentions in recent years because of their excellent surface activity, good environment compatibility, biodegradability, and unique bioactivities [1]. As one of the most important biosurfactants, SLs has good biocompatibility and biodegradability and the highest fermentation production among all of biosurfactants [2]. SLs is an environmental friendly biosurfactant that can be used in food and daily chemicals, medical care, environment protection, microbial enhanced oil recovery, pesticides, and many other industrial and agricultural fields [3]. The obstacle to the large-scale applications of SLs lies in the higher production cost of SLs over chemically synthesized surfactants. High production of SLs can be achieved by adding both high concentration of hydrophilic (up to 80 g/L of glucose) and hydrophobic (such as 60 g/L rapeseed oil) carbon sources; thus, the cost of the carbon sources accounts for a large proportion of the total cost of SL production and is a major bottlene
Data Loading...
