Peculiarities of Cell Seeding on Electroformed Polycaprolactone Scaffolds Modified with Surface-Active Agents Triton X-1
- PDF / 2,054,475 Bytes
- 5 Pages / 594 x 792 pts Page_size
- 95 Downloads / 179 Views
600
Cell Technologies in Biology and Medicine, No. 2, August, 2020
Peculiarities of Cell Seeding on Electroformed Polycaprolactone Scaffolds Modified with Surface-Active Agents Triton X-100 and Polyvinylpyrrolidone
S. A. Afanasiev1, E. F. Muslimova1, Yu. A. Nashchekina2, P. O. Nikonov2, Yu. V. Rogovskaya1, T. Kh. Tenchurin3, E. V. Nesterenko3, E. V. Grakova1, K. V. Kopeva1, and S. D. Akhmedov1 Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 143-148, June, 2020 Original article submitted February 17, 2020 We compared the capability of human fibroblasts to populate porous polycaprolactone (PCL) scaffolds modified during fabrication with surface-active agents Triton Х-100 (type 1 scaffold) and polyvinylpyrrolidone (type 2 scaffold). The mean fiber diameter in both scaffolds was almost the same: 3.90±2.19 and 2.46±2.15 µ, respectively. Type 1 scaffold had higher surface density and hydrophilicity, when type 2 scaffold was 1.6 times thicker. The cells were seeded on the scaffolds by the dynamic seeding technique and then cultured in Petri dishes with nutrient medium in a humid atmosphere. During 3-day culturing, no cell release from the matrix was noted. DAPI staining proved the presence of cells in both scaffolds. However, in type 1 scaffold the cells populated the whole thickness, while in type 2 scaffold, the cells were present only in the superficial layer. These findings suggest that PCL scaffolds modified with Triton Х-100 or polyvinylpyrrolidone are not cytotoxic, but the structure of the scaffold treated with Triton Х-100 is more favorable for population with cells. Key Words: tissue engineering; synthetic scaffold; Triton Х-100; polyvinylpyrrolidone; cell culture The progress in regenerative medicine and in fabrication of artificial tissues and organs for substitutive surgery depends, among other things, on the development of available 3D structures for cell seeding [4,6]. Fabrication of scaffolds similar to biological tissues by their 3D structure is the most promising approach [11]. The problem of fabrication of these scaffolds is largely solved due to the development of electrospinning technology [1,5]. This method allows producing fibrous scaffolds from various synthetic materials with predetermined structural characteristics and strength properties close to biological tissues [5]. The need in Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk; 2Institute of Cytology, Russian Academy of Sciences, St. Petersburg; 3National Research Center “Kurchatov Institute”, Moscow, Russia. Address for correspondence: [email protected]. S. A. Afanasiev 1
scaffolds promoting more efficient population with cells and preserving their proliferative activity and the capacity to direct differentiation steadily increases [8]. A promising approach to improving scaffold characteristics is addition of bioactive or surface-active agents [5] to the basic substance used for scaffold fabrication; these additives can essentially modify the properti
Data Loading...
