Photochemical Micro-pattern Substitution of Functional Groups for Protein Attachment Control
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0950-D06-04
Photochemical Micro-Pattern Substitution of Functional Groups for Protein Attachment Control Masataka Murahara1 and Yuji Sato2 1
Entropia Laser Initiative, Tokyo Institute of Techonology, P.O.Box I3-25, 2-12-1, O-okayama, Megro-ku, Tokyo, 152-8552, Japan
2
Integrated Research Institute, Tokyo Institute of Techonology, Meguro-ku Tokyo, 152-8552, Japan
ABSTRACT Hydrophilic and hydrophobic groups were selectively incorporated on the poly(methyl methacrylate) [PMMA] surface using a Xe2 excimer lamp and ArF excimer laser. With this new technique, a protein adsorption on the PMMA surface can be controlled. PMMA was firstly irradiated with a Xe2 excimer lamp in the presence of perfluoropolyether [PFPE] liquid layer to incorporate CF3 groups, and secondly, the PMMA surface was irradiated by an ArF excimer laser through a patterned reticle in the presence of water to incorporate OH groups or NH2 groups in an ammonia gas ambience. The area ratio of hydrophilic and hydrophobic of the modifying sample was made to 1:3, 1:1, and 3:1. The results showed that the fibrin absorption on the sample with hydrophilic and hydrophobic micro domains depended on the area ratio of the hydrophilic and hydrophobic. The absorption coefficient of the amide band remarkably decreased with increase in water contact angle. Furthermore, it was confirmed that the absorption coefficient of fibrin decreased as the interval of CF3 and OH or NH2 groups was narrowed from 250 to 20 µm, and the fibrin sticking on the modified surface with the 20 µm hydrophilic and hydrophobic micro domains was reduced to one-twenty of that on the untreated sample. 1.
INTRODUCTION
Plastics are needed for artificial organ materials into a living body. In general, protein adheres to the material surface when a foreign matter is implanted to the living body, where fibroblast or tissue cell is proliferated. And a foreign-body reaction or an immune reaction occurs in the interface with the biological tissue. To prevent this reaction, namely histoincompatibility, fluorocarbon polymers, acrylic resin, polypropylene, and polyethylene terephthalate are widely used for clinical application. Fluorocarbon polymers, among them, show a high resistance to chemicals and an excellent weathering, and are inactive in the living body; which are used for artificial heart, artificial lung, artificial fascia, artificial dura mater, artificial ligament, artificial joint, artificial vessel, and surgical suture. However, these materials for hindering the rejection are not biocompatible in true meaning. A hydrophilic property is essential for the biocompatibility. Material surface modifications designed for high biocompatibility have been studied by using various methods very often in recent years1. To improve the biocompatibility, Migonney et al 2 modified the silicone contact lens to incorporate the methyl groups, epoxy groups, or carboxyl groups by chemical treatment. Imai and Watanabe3
modified the wettability of an ethylene-vinyl alcohol copolymer film by soaking the sample in
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