Photoelectrochemical immunoassay platform based on MoS 2 nanosheets integrated with gold nanostars for neuron-specific e
- PDF / 1,081,432 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 25 Downloads / 202 Views
ORIGINAL PAPER
Photoelectrochemical immunoassay platform based on MoS2 nanosheets integrated with gold nanostars for neuron-specific enolase assay Renxi Liu 1 & Yanying Wang 1 & Wingleung Wong 2,3 & Haiyan Li 1 & Chunya Li 1 Received: 22 January 2020 / Accepted: 23 June 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract MoS2 nanosheets were prepared by exfoliating MoS2 bulk crystals with ultrasonication in N-methylpyrrolidone and were integrated with gold nanostars (AuNS) to fabricate an AuNS/MoS2 nanocomposite. All nanomaterials were characterized by transmission electron microscope, scanning electron microscope, ultraviolet-visible spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. AuNS/MoS2 nanocomposites were coated onto a glassy carbon electrode (GCE) surface to construct a nanointerface for immobilizing neuron-specific enolase antibody (anti-NSE) thus forming a photoelectrochemical immunoassay system. AuNS can significantly promote the photoelectric conversion of MoS2 nanosheets improving the performance for a photoelectrochemical assay. Being illuminated with white light LED and controlling the potential at 0.05 V (vs. SCE), the photocurrent generated from anti-NSE(BSA)/AuNS/MoS2/GCE using 0.15 mol L−1 ascorbic acid as electron donor can be recorded with amperometry and used as an output signal for NSE quantitative assay. Under optimized experimental conditions, the photocurrent variation for the affinity-binding NSE is proportional to the logarithm of NSE concentration in the range 5.0 pg mL-1 to 1.5 ng mL−1 with a detection limit of 3.5 pg mL−1 (S/N = 3). The practicability of the PEC immunoassay system was evaluated by determining NSE in clinical serum samples. The recoveries ranged from 93.0 to 103% for the determination of NSE in serum samples with a standard addition method. The PEC immunoassay system possesses good accuracy for determining NSE in real samples.
Keywords Photoelectrochemical immunoassay . Neuron-specific enolase . MoS2 nanosheet . Gold nanostar
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04411-7) contains supplementary material, which is available to authorized users. * Haiyan Li [email protected] * Chunya Li [email protected] 1
Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education & Hubei Key Laboratory of Catalysis and Materials Science & Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, South-Central University for Nationalities, Wuhan 430074, China
2
School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, China
3
International Healthcare Innovation Institute (Jiangmen), Jiangmen 529040, China
Lung cancer has been considered as a good indicator for evaluating global cancer mortality. Neuron-specific enolase (NSE) as a well-known tumor marker is very specific for the early diagnosis of small cell lung carcinoma (SCLC) [1]. As patients suffering from some tumor
Data Loading...
