Phylogenomic Analysis Reveals That Arthrobacter mysorens Nand and Rao 1972 (Approved Lists 1980) and Glutamicibacter mys
- PDF / 1,100,043 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 80 Downloads / 181 Views
Phylogenomic Analysis Reveals That Arthrobacter mysorens Nand and Rao 1972 (Approved Lists 1980) and Glutamicibacter mysorens Busse 2016 are Later Heterotypic Synonyms of Arthrobacter nicotianae Giovannozzi‑Sermanni 1959 (Approved Lists 1980) and Glutamicibacter nicotianae Busse 2016 Sushanta Deb1 · Lipika Das1 · Subrata K. Das1 Received: 19 January 2020 / Accepted: 21 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract We described the comparative genomic analysis to delineate the taxonomic relationship between the two Glutamicibacter species Glutamicibacter mysorens NBRC 103060T and Glutamicibacter nicotianae NBRC 14234T. Phylogenetic tree based on concatenated ribosomal protein marker genes showed both species clade together. The average nucleotide identity (ANI) values between G. mysorens NBRC 103060T and G. nicotianae NBRC 1 4234T ranged from 97.23 to 97.97%. Further, the average amino acid identity (AAI) between two strains were more than 97.61%. The ANI, AAI and in silico DNA–DNA hybridization (isDDH) data were higher than the threshold value for bacterial species delineation. The two strains have identical profile of fatty acids, sugars and lipid composition; and overall similar phenotypic characteristics. It therefore becomes evident that the two species actually belong to the same species. Arthrobacter nicotianae (Giovannozzi-Sermanni 1959) (Approved Lists 1980) and Glutamicibacter nicotianae Busse 2016 have priority over the names Arthrobacter mysorens (Nand and Rao 1972) (Approved Lists 1980) and Glutamicibacter mysorens Busse 2016, therefore we proposed that Glutamicibacter mysorens Busse 2016 is a later heterotypic synonym of Glutamicibacter nicotianae Busse 2016. The type strain is ATCC 1 5236T = CCM 1648T = BCRC (formerly CCRC) 1 1219T = CCUG 23842T = CDA 883T = CIP 82.107T = DSM 20123T = HAMBI 1859T = IAM 12342T = IFO (now NBRC) 1 4234T = IMET 10353T = JCM 1333T = LMG 16305T = NCIMB 9458T = NRIC 0153T.
Introduction Recently, the species of only genus Arthrobacter have been reclassified as species of the genus Glutamicibacter based on phylogenetic and chemotaxonomic analysis [1, 2]. Moreover, phylogeny using concatenated ribosomal protein coding sequences along with evaluation of average nucleotide identity (ANI) and in silico DDH are being used for the reclassification of several bacterial taxa [3, 4]. It had been possible Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02176-z) contains supplementary material, which is available to authorized users. * Subrata K. Das [email protected]; [email protected] 1
Department of Biotechnology, Institute of Life Sciences, Nalco Square, Bhubaneswar 751023, India
by whole genome sequencing of microorganisms and their comparative phylogenomic analysis. Busse [1] described the emendation of the genus Arthrobacter and transfer of Arthrobacter mysorens [5] and Arthrobacter nicotianae [6] to the genus Glutamicibacter and the type species
Data Loading...
