Multiple omics analysis reveals that high fiber diets promote gluconeogenesis and inhibit glycolysis in muscle
- PDF / 2,481,130 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 20 Downloads / 165 Views
RESEARCH ARTICLE
Open Access
Multiple omics analysis reveals that high fiber diets promote gluconeogenesis and inhibit glycolysis in muscle Jianghong Wu1,2* , Ding Yang2, Husile Gong3, Yunxia Qi2, Hailian Sun2, Yongbin Liu2, Yahong Liu2 and Xiao Qiu2
Abstract Background: Meat quality is a complex trait affected by genotypic and environmental factors. In a previous study, it was found that feedstuffs have various effects on the growth rate and meat quality of lambs. However, the underlying mechanisms are still not entirely clear. Results: In this study, to investigate the mechanisms that impact meat quality in twin sheep fed either with high fiber low protein (HFLP) forage (Ceratoides) or low fiber high protein (LFHP) forage (alfalfa) diets, multi omics techniques were utilized for integration analysis based on the feed nutritional value and the sheep microbiome, transcriptome, metabolome, and fatty acid profile. Results showed that the production performance and the muscle components of lambs were significantly affected by feeds. The essential fatty acid (linoleic acid and arachidonic acid) content of the muscle, based on gas chromatography-mass spectrometry analysis, was increased when lambs were fed with HFLP. The microbes in the lambs’ rumen fed a HFLP diet were more diverse than those of the LFHP fed group. Besides, the ratio of Bacteroidetes and Firmicutes in the rumen of the sheep fed a LFHP diet was 2.6 times higher than that of the HFLP fed group. Transcriptome analysis of the muscle revealed that the genes related to glucose metabolic processes and fatty acid biosynthesis were significantly differentially expressed between the two groups. Potential cross talk was found between the sfour omics data layers, which helps to understand the mechanism by which feedstuffs affect meat quality of lambs. Conclusion: Feed systems may affect the epigenetic regulation of genes involved in the glucose metabolic pathway. HFLP feeds could induce gluconeogenesis to maintain glucose levels in blood, resulting in decreased fat content in muscle. The multiple omics analysis showed that the microbiota structure is significantly correlated with the metabolome and gene expression in muscle. This study laid a theoretical foundation for controlling the nutrient intake of sheep; it suggested that its fatty acid spectrum modifications and the removal of meat quality detrimental material could guide sheep feeding for functional mutton. Keywords: Glucose metabolism, Integrative analysis, Meat quality, Metabolome, Ruminal microbiome, Transcriptome
* Correspondence: [email protected] 1 College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China 2 Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution an
Data Loading...
