Plasma and urinary inositol isomer profiles measured by UHPLC-MS/MS reveal differences in scyllo-inositol levels between
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RESEARCH PAPER
Plasma and urinary inositol isomer profiles measured by UHPLC-MS/MS reveal differences in scyllo-inositol levels between non-pregnant and pregnant women Irina Monnard 1 & Thierry Bénet 1 & Rosemarie Jenni 1 & Sean Austin 1 & Irma Silva-Zolezzi 2 & Jean-Philippe Godin 1 Received: 20 May 2020 / Revised: 17 July 2020 / Accepted: 27 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Previous studies support that myo- and D-chiro-inositol isomers are promising bioactives for the treatment of women with polycystic ovary syndrome and for lowering the risk of gestational diabetes mellitus in pregnant women, whereas scyllo-inositol may have some benefits for neurological disorders (e.g., Alzheimer’s disease). Though potentially useful to better understand inositol isomer metabolism and study their role in health and disease, routine analysis of inositol isomers in plasma and urine with a single analytical method is not yet feasible due to the lack of a suitable analytical assay. To address this, we developed and validated a robust ultra-high-performance-liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for the quantification of inositol isomers in plasma and urine. This method resolves seven inositol isomers with accurate quantification of total chiro- (D and L enantiomers), myo-, and scylloinositols and is semi-quantitative for neo-inositol. For urine and plasma myo-inositol, the method repeatability and intermediate reproducibility were below 6% and 8%, respectively. Then, for both chiro- and scyllo-inositols, repeatability and intermediate reproducibility were below 10% and 14%, respectively. A pilot study was carried out to quantify and compare the pattern of inositol isomers in urine and plasma of non-pregnant and pregnant women and showed for the first time that urinary myo- and scyllo-inositol concentrations were significantly higher for women in the third trimester of pregnancy compared with non-pregnant women. These findings warrant further research to understand the biological significance of the observed differences in inositol profiles and suggest a potential role of scyllo-inositol. Keywords Myo-inositol . Scyllo-inositol . Neo-inositol . UHPLC-MSMS . Plasma . Urine
Introduction Chemically, inositol isomers are cyclohexane-1,2,3,4,5,6hexol with a molecular weight of 180 Da. Nine inositol structural stereoisomers exist, and among them, eight are naturally
Irina Monnard and Thierry Bénet contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00216-020-02919-8) contains supplementary material, which is available to authorized users. * Jean-Philippe Godin [email protected] 1
Institute of Food Safety and Analytical Sciences, Nestlé Research, Vers-chez-les Blanc, 1000 Lausanne 26, Switzerland
2
Nestlé Research, Singapore Hub, 29 Quality Road, Singapore 618802, Singapore
occurring (either in plants, mammals, or bacteria). They are named scyllo-, muco-, epi-
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