Pleiotropic QTL influencing spikelet number and heading date in common wheat ( Triticum aestivum L.)
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ORIGINAL ARTICLE
Pleiotropic QTL influencing spikelet number and heading date in common wheat (Triticum aestivum L.) Zhaoyan Chen1,2 · Xuejiao Cheng1,2 · Lingling Chai1,2 · Zihao Wang1,2 · Dejie Du1,2 · Zhihui Wang1,2 · Ruolin Bian1,2 · Aiju Zhao3 · Mingming Xin1,2 · Weilong Guo1,2 · Zhaorong Hu1,2 · Huiru Peng1,2 · Yingyin Yao1,2 · Qixin Sun1,2 · Zhongfu Ni1,2 Received: 17 September 2019 / Accepted: 24 January 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Three pleiotropic QTL regions associated with spikelet number and heading date were identified, with FT-A1 considered the candidate gene for QTspn/Hd.cau-7A. Abstract Spikelet number traits and heading date (HD) play key roles in yield improvement of wheat and its wide adaptation to different environments. Here, we used a Recombinant Inbred Lines population derived from a cross between Yi5029 (5029) and Nongda4332 (4332) to construct a high-density genetic linkage map and identify quantitative trait loci (QTL) associated with total spikelet number per spike (TSPN), fertile spikelet number per spike (FSPN), sterile spikelet number per spike (SSPN) and HD. A total of 22 environmentally stable QTL for TSPN, FSPN, SSPN and HD were identified. Notably, three pleiotropic QTL regions for TSPN and HD were detected on chromosomes 2A, 7A and 7D. The QTL associated with TSPN and HD on chromosome 7AS was designated QTspn/Hd.cau-7A. Furthermore, the candidate gene FT-A1 located in the region of QTspn/Hd.cau-7A had a single-nucleotide polymorphism (T–G) within the third exon, which might be the cause of diversity in spikelet number and HD between the two parents. Additionally, we developed a semi-thermal asymmetric reverse PCR (STARP) marker to analyze the geographical distribution and evolution of FT-A1 (T or G) alleles. This study contributes to our understanding of the molecular mechanisms of the four traits (TSPN, FSPN, SSPN and HD) and provides further insights into the genetic relationship between spikelet number traits and HD in wheat.
Introduction
Communicated by Albrecht E. Melchinger. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03556-6) contains supplementary material, which is available to authorized users. * Zhongfu Ni [email protected] 1
State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
2
National Plant Gene Research Centre, Beijing 100193, China
3
Hebei Crop Genetic Breeding Laboratory, Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, China
Wheat (Triticum aestivum L.) is one of the most important crops for human and animal nutrition and is produced across a large part of the world (Case histories: Crops, FAO 2010). Therefore, increasing wheat yield will be essential for ensuring future food security, given the dual challenges of an incr
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