Polycaprolactone-gelatin nanofibers incorporated with dual antibiotic-loaded carboxyl-modified silica nanoparticles
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Polycaprolactone-gelatin nanofibers incorporated with dual antibiotic-loaded carboxyl-modified silica nanoparticles Zahra Gounani1,2,3,4, Sajedeh Pourianejad2,6, Mohammad Ali Asadollahi2,* Rikke L. Meyer4,5, Jessica M. Rosenholm3, and Ayyoob Arpanaei1,*
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1
Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, P. O. BOX 1417863171, Iran 2 Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan 8174673441, Iran 3 Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering, Åbo Akademi University, 20520 Turku, Finland 4 Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus, Denmark 5 Department of Bioscience, Aarhus University, 8000 Aarhus, Denmark 6 Joint School of Nanoscience and Nanoengineering, University of North Carolina in Greensboro, Greensboro, USA
Received: 7 April 2020
ABSTRACT
Accepted: 1 September 2020
In this study, we used electrospun polycaprolactone (PCL) or a mixture of PCL and gelatin (Gel) in a mixed acidic solvent to develop antimicrobial electrospun nanofibers. Carboxyl-modified mesoporous silica nanoparticles (CMSNs) or CMSNs loaded with antibiotic drugs polymyxin B and vancomycin (CMSNs/ ABs) were mixed with the electrospinning solution in concentrations of 1%, 2.5% and 5%. The nanofibers diameter measured between 122 and138 nm. Higher concentrations of gelatin or CMSNs increased hydrophilicity and degradability of the nanofibers. CMSNs enhanced nanofibers mechanical strength. PCL/Gel nanofibers incorporated with CMSNs/ABs (2.5% and 5%) showed high antibacterial efficiency against Pseudomonas aeruginosa and Staphylococcus aureus. Also bacterial cell adhesion decreased when 2.5% and 5% of CMSNs/ABs were incorporated in PCL/Gel mats. MTT and hemolysis assays indicated excellent biocompatibility of all types of electrospun nanofibers. This study confirms that a proper mixture of PCL, gelatin and CMSNs loaded with two antibiotics could offer antimicrobial activities with high biocompatibility and biodegradability properties.
Published online: 21 September 2020
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Springer Science+Business
Media, LLC, part of Springer Nature 2020
Handling Editor: Annela M. Seddon.
Address correspondence to E-mail: [email protected]; [email protected]
https://doi.org/10.1007/s10853-020-05253-7
17135
J Mater Sci (2020) 55:17134–17150
GRAPHIC ABSTRACT
Introduction Bacterial infections involving biofilms are one of the most challenging issues for both acute and chronic wounds [1]. Wound infections are mostly polymicrobial infections involving numerous bacterial strains such as Escherichia coli, Acinetobacter
calcoaceticus, Klebsiella pneumonia, Acinetobacter baumannii, Enterobacter cloacae and the most common species Pseudomonas aeruginosa and Staphylococcus aureus [2]. Some commonly used antibiotics for wound infections include penicillin G, gentamicin, bacitracin, metronidazole, polymyxin B and vancomycin [3, 4]. Vancomycin is effective aga
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