Polydopamine-mediated Surface Modification Promotes the Adhesion and Proliferation of Human Induced Pluripotent Stem Cel
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MRS Advances © 2019 Materials Research Society DOI: 10.1557/adv.2019.405
Polydopamine-mediated Surface Modification Promotes the Adhesion and Proliferation of Human Induced Pluripotent Stem Cells Yan Niea,b, Zijun Denga,c, Weiwei Wanga, Thanga Bhuvanesha,b, Nan Maa,c,d* and Andreas Lendleina,b,c,d* aInstitute
of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513, Teltow, Germany
bInstitute
of Biochemistry and Biology, University of Potsdam, 14476, Potsdam, Germany
cInstitute
of Chemistry and Biochemistry, Free University of Berlin, 14195, Berlin, Germany
dHelmholtz
Virtual Institute − Multifunctional Materials in Medicine, Berlin and Teltow, Germany
* To whom correspondence should be addressed: Prof. Dr. Nan Ma, email: [email protected] Prof. Dr. Andreas Lendlein, email: [email protected]
ABSTRACT
With their abilities of self-renewal and pluripotency to differentiate into all three germ layers, human induced pluripotent stem cells (hiPSCs) are a promising cell source for cell-based drug and implant testing. However, the large-scale expansion and maintenance of hiPSCs requires following strict protocols. There is high demand for advanced cell culture systems capable of generating high-quality hiPSCs to meet application requirements. In this study, we probe the possibility of modifying polymeric substrates for maintaining the self-renewal and pluripotency of hiPSCs. Here, polydopamine (PDA) was employed to immobilize the Laminin 521 (LN521) onto the surface of polyethylene terephthalate (PET). An aqueous solution of dopamine with concentrations ranging from 0 to 2.0 mg/mL was applied on PET surfaces. These PDAmodified surfaces were further functionalized with LN521. Surface wettability was
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evaluated by measuring the water contact angle (WCA) and surface properties of the modified substrate were analyzed using an atomic force microscope (AFM). Initial hiPSC attachment (1h after seeding) and cell proliferation were evaluated by counting the total cell number. The maintenance of pluripotency was evaluated at designed time points. WCA of the PDA-LN521 surfaces gradually decreased from 62.1°±6.3° to 8.1°±2.9°. The maximum peak-to-valley height roughness (Rt) of those surfaces determined by AFM increased in a dopamine-concentration-dependent manner, ranging from 43.9±1.6 nm to 126.7±7.6 nm. The Young's modulus of these surfaces was substantially increased from 0.98±0.36 GPa to 4.81±2.41 GPa. There was a significant enhancement (13.0±7.2% and 24.2±8.1%) of hiPSC adhesion on PDA-LN521 (dopamine concentration at 0.125 and 0.25 mg/mL). When increasing the dopamine concentration to 0.5 and 1.0 mg/mL, there was no further increase in hiPSC adhesion on PDA-LN521 surfaces. Moreover, hiPSC proliferation was remarkably enha
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