Polymorphism analysis and supertype definition of swine leukocyte antigen class I molecules in three-way crossbred (Land
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lymorphism analysis and supertype definition of swine leukocyte antigen class I molecules in three-way crossbred (Landrace, Duroc, and Yorkshire) pigs: implications for the vaccine development of African swine fever virus 1,2
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Limin Ba , Zhenbao Wang , William J Liu , Dongxun Wu , Wangzhen Xiang , Peng Qi , 2 5 6 2* 1* Chunna Dong , Yanxin Hu , Ping Lu , Jin Xiao & Changyuan Yu 1
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China; 2 Zhongmu Research Institute, China Animal Husbandry Industry Co., LTD, Beijing 100095, China; 3 China Animal Husbandry Industry Co., LTD, Beijing 100095, China; 4 National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China; 5 Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China; 6 China Animal Health and Epidemiology Center, Qingdao 266032, China Received March 4, 2020; accepted April 21, 2020; published online May 11, 2020
Citation:
Ba, L., Wang, Z., Liu, W.J., Wu, D., Xiang, W., Qi, P., Dong, C., Hu, Y., Lu, P., Xiao, J., et al. (2020). Polymorphism analysis and supertype definition of swine leukocyte antigen class I molecules in three-way crossbred (Landrace, Duroc, and Yorkshire) pigs: implications for the vaccine development of African swine fever virus. Sci China Life Sci 63, https://doi.org/10.1007/s11427-019-1696-7
Dear Editor, Swine major histocompatibility complex (MHC) is a highly polymorphic gene in pigs and is also called swine leukocyte antigen (SLA) (Fan et al., 2018). SLA is divided into three major categories, SLA I (SLA-1, -2, -3), SLA II, and SLA III (Smith et al., 2005). SLA I plays an important role in cellular immunity which can eliminate viruses and other foreign antigens in pigs (Macdonald et al., 2010). SLA-1, SLA-2 and SLA-3 are important SLA I alleles and are highly polymorphic. Polymorphism causes extreme difficulties in screening broadly protective cytotoxic lymphocyte (CTL) epitope antigens. The supertype which is built based on peptide-binding specificity of each SLA I can overcome this difficulty to some extent (Thomsen et al., 2013). Three-way crossbred (Landrace, Duroc, and Yorkshire) pigs are widely raised in almost every pig farm in China (approximately 600 million pigs per year). To develop vaccines for this strain of *Corresponding authors (Changyuan Yu, email: [email protected]; Jin Xiao, email: [email protected])
pigs, SLA I alleles and the supertype of SLA I in these threeway crossbred pigs must be studied. In this study, a total of 164 novel SLA I alleles were identified from three-way crossbred pigs. Detailed procedures of identifying the SLA I alleles are available in supplementary file. We submitted the nucleotide sequences of these new SLA I alleles to the GenBank database and obtained accession numbers: MH294957-MH295122 (Table S1 in Supporting Information). The SLA I allele database is enriched. We have pres
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