Positional cloning of a candidate gene for resistance to the sunflower downy mildew, Plasmopara halstedii race 300
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ORIGINAL PAPER
Positional cloning of a candidate gene for resistance to the sunflower downy mildew, Plasmopara halstedii race 300 Je´roˆme Franchel • Mohamed Fouad Bouzidi • Gise`le Bronner • Felicity Vear • Paul Nicolas • Said Mouzeyar
Received: 12 March 2012 / Accepted: 15 September 2012 / Published online: 29 September 2012 Ó Springer-Verlag Berlin Heidelberg 2012
Abstract The resistance of sunflower to Plasmopara halstedii is conferred by major resistance genes denoted Pl. Previous genetic studies indicated that the majority of these genes are clustered on linkage groups 8 and 13. The Pl6 locus is one of the main clusters to have been identified, and confers resistance to several P. halstedii races. In this study, a map-based cloning strategy was implemented using a large segregating F2 population to establish a fine physical map of this cluster. A marker derived from a bacterial artificial chromosome (BAC) clone was found to be very tightly linked to the gene conferring resistance to race 300, and the corresponding BAC clone was sequenced
Communicated by A. Berville´.
Electronic supplementary material The online version of this article (doi:10.1007/s00122-012-1984-6) contains supplementary material, which is available to authorized users. J. Franchel M. F. Bouzidi F. Vear P. Nicolas S. Mouzeyar (&) Clermont Universite´, Universite´ Blaise Pascal, UMR INRA-UBP 1095 GDEC, BP 10448, 63000 Clermont-Ferrand, France e-mail: [email protected] J. Franchel Clermont Universite´, Universite´ Blaise Pascal, UMR 547 PIAF, BP 10448, 63000 Clermont-Ferrand, France J. Franchel INRA, UMR 547 PIAF, 63100 Clermont-Ferrand, France G. Bronner Clermont Universite´, Universite´ Blaise Pascal, Laboratoire ‘‘Microorganismes: Ge´nome et Environnement’’, BP 10448, 63000 Clermont-Ferrand, France G. Bronner CNRS, UMR 6023, LMGE, 63171 Aubiere, France
and annotated. It contains several putative genes including three toll-interleukin receptor-nucleotide binding site-leucine rich repeats (TIR-NBS-LRR) genes. However, only one TIR-NBS-LRR appeared to be expressed, and thus constitutes a candidate gene for resistance to P. halstedii race 300.
Introduction Sunflower downy mildew is caused by the obligate parasitic oomycete, Plasmopara halstedii. The resistance of sunflower to this parasite is controlled by single dominant genes denoted Pl (Vranceanu and Stoenescu 1971), each being effective against one of the many races that have been described worldwide. Genetic studies have shown that the interaction between sunflower and downy mildew races probably complies with the gene for gene concept as initially described by Flor (1947). Pl1 was the first Pl gene to be localised on linkage group 8 of the sunflower map (Mouzeyar et al. 1995). Other Pl genes were later mapped and proved to be clustered (Vear et al. 1997; Bachlava et al. 2011). Sequence comparisons of the plant resistance genes that have been cloned show that the majority of these genes belong to a highly represented gene family containing two domains; a nucleot
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