Potential utilization of a lambda carrageenan polysaccharide, derived from a cultivated, clonal strain of the red seawee
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Potential utilization of a lambda carrageenan polysaccharide, derived from a cultivated, clonal strain of the red seaweed Chondrus crispus (Irish moss) against toxic actions of venom of Bothrops jararaca and B. jararacussu snakes Ana Cláudia Rodrigues da Silva 1 & Kelly Ketely Granja Pereira 1 & Alan Trevor Critchley 2 & Eladio Flores Sanchez 3 & André Lopes Fuly 1 Received: 25 March 2020 / Revised and accepted: 13 August 2020 # Springer Nature B.V. 2020
Abstract Snakebites are a serious occupational problem affecting rural populations of tropical and sub-tropical, developing countries. Envenomation caused by the snakes Bothrops jararaca and B. jararacussu is characterized by local pain, edema, hemorrhage, tissue necrosis, and death. Despite the fact that antivenom may prevent the death of bite victims, treatment does not prevent tissue necrosis, often leading to amputation or deformity of the victim’s affected limb.Therefore, more efficient therapies need to be investigated. In this work, we tested the ability of a carrageenan galactan polysaccharide, isolated from a clonal strain of the red alga, Chondrus crispus (commonly known as Irish moss) to inhibit toxic, in vitro (coagulation, hemolytic and proteolytic) or in vivo (hemorrhagic, edematogenic, myotoxic and lethal) activities of B. jararaca or B. jararacussu venom. When the polysaccharide was mixed together with the venoms, inhibition of their toxic activities was achieved, but with different potencies. Moreover, inhibition of hemorrhage, edema, lethality, or myotoxicity was observed, even if the polysaccharide was injected before or after the injection of venoms, regardless of the route of administration (i.e., intravenous, subcutaneous, and intraperitoneal). A gel formulation containing the polysaccharide of C. crispus also protected mice from hemorrhage after administration of the venoms. Thus, the lambda carrageenan polysaccharide, as produced by a strain of C. crispus, may aid antivenom to block the toxic activities of B. jararaca and B. jararacussu venom, as well as to aid in the development of a more efficient therapy for envenomation by these venomous snakes. Keywords Bothrops jararaca . Bothrops jararacussu . Chondrus crispus . Rhodophyta . Lambda carrageenan . Antivenom
* André Lopes Fuly [email protected] Ana Cláudia Rodrigues da Silva [email protected] Kelly Ketely Granja Pereira [email protected] Alan Trevor Critchley [email protected] Eladio Flores Sanchez [email protected] 1
Department of Molecular and Cellular Biology, Federal Fluminense University, Niterói, Rio de Janeiro 24020-141, Brazil
2
Verschuren Centre for Sustainability in Energy and Environment, University of Cape Breton, Nova Scotia, Canada
3
Laboratory of Biochemistry of Proteins from Animal Venoms, Research and Development Center, Ezequiel Dias Foundation, Belo Horizonte, Minas Gerais 30510-010, Brazil
Introduction Snakebite envenoming is a neglected tropical affliction resulting from the injection of a highly specialized toxic secretion, usu
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