Paenibacillus panacihumi sp. nov., a potential plant growth-promoting bacterium isolated from ginseng-cultivated soil
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ORIGINAL PAPER
Paenibacillus panacihumi sp. nov., a potential plant growth-promoting bacterium isolated from ginseng-cultivated soil Chun‑Gon Kim1 · Jong‑Pyo Kang1 · Yue Huo1 · Mohan Chokkalingam1 · Yeon‑Ju Kim1 · Dong‑Hyun Kim1 · Deok‑Chun Yang1,2 Received: 26 December 2017 / Revised: 10 April 2018 / Accepted: 12 April 2018 © Springer-Verlag GmbH Germany, part of Springer Nature 2018
Abstract A novel bacterium, designated D CY114T, was isolated from ginseng-cultivated soil in Gochang-gun, Republic of Korea. This isolate was assigned to the genus Paenibacillus and is closely related to Paenibacillus amylolyticus NRRL NRS-290T (98.3%), P. dongdonensis KUDC0114T (98.0%), P. tylopili MK2T (97.9%), P. tundrae A10bT (97.8%), and P. xylanexedens B22aT (97.5%) based on 16S rRNA gene sequence analysis. Strain D CY114T is a Gram-reaction positive, catalase and oxidase positive, facultatively aerobic rod that is motile by peritrichous flagella. Strain D CY114T produces siderophores and indole-3-acetic acid (IAA) and is able to solubilize phosphate as a plant growth-promoting bacterium. MK-7 was the diagnostic menaquinone. The major cellular fatty acids were anteiso-C15:0, C16:0, and C18:0, and the major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), and an unknown amino lipid (AL1,2). The genomic DNA G + C content was 46.0 mol%. Phenotypic and chemotaxonomic results also placed strain DCY114T within the genus Paenibacillus. DNA–DNA homology values between strain DCY114T and closely related reference strains were lower than 43%. The low DNA relatedness data in combination with phylogenetic and biochemical tests indicated that strain DCY114T could not be assigned to a recognized species. The results of this study support that the DCY114T strain is a novel species belonging to the genus Paenibacillus, for which the name Paenibacillus panacihumi is proposed. The type strain is DCY114T (= KCTC 33915T = JCM 32073T). Keywords Paenibacillus panacihumi · Ginseng-cultivated soil · Plant growth-promoting bacterium
Communicated by Erko Stackebrandt. Chun-Gon Kim and Jong-Pyo Kang are contributed equally to this work. The NCBI GenBank accession number for the 16S rRNA gene sequence of strain DCY114T is KY694399. DPD (digital protologue’ database) Taxon number: TA00398. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-018-1517-z) contains supplementary material, which is available to authorized users. * Yeon‑Ju Kim [email protected] * Deok‑Chun Yang [email protected] 1
Department of Oriental Medicinal Biotechnology, Kyung Hee University, Seocheon‑dong, Giheung‑gu, Yongin‑si, Gyeonggi‑do 446‑701, Republic of Korea
Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Seocheon‑dong, Giheung‑gu, Yongin‑si, Gyeonggi‑do 446‑701, Republic of Korea
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Introduction The genus Paenibacillus of the family Paenibacillaceae was proposed by Ash et al. (1993) and later amended by Shida et al. (1997). Presen
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