Structure and anticancer activity of a new lectin from the cultivated red alga, Kappaphycus striatus
- PDF / 2,670,146 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 17 Downloads / 172 Views
NOTE
Structure and anticancer activity of a new lectin from the cultivated red alga, Kappaphycus striatus Le Dinh Hung1 · Phan Thi Hoai Trinh1 Received: 10 February 2020 / Accepted: 27 September 2020 © The Japanese Society of Pharmacognosy 2020
Abstract The red alga Kappaphycus striatus is economically important food species and extensively cultivated throughout most tropical parts of the world as a source of carrageenan. In this note, the primary structure of a new lectin KSL from this alga was elucidated by the rapid amplification method of complementary DNA (cDNA) ends, which consists of 267 amino acid residues distributed in four tandem-repeated domains of about 67 amino acids and sharing 43% of identity. The calculated molecular mass from the deduced sequence was consistent with that of natural KSL (27,826 Da) determined by electron spray ionization–mass spectrometry. The primary structure of KSL showed high similarity to those of the high mannose N-glycan specific lectins from marine red algae, ESA-2 from Eucheuma serra, EDA-2 from Eucheuma denticulatum, KSA-2 from Kappaphycus striatum, KAAs from Kappaphycus alvarezii and SfLs from Solieria filiformis, and from microorganisms, BOA from Burkholderia oklahomensis, MBHA from Myxococcus xanthus, OAA from Oscillatoria agardhii and PFL from Pseudomonas fluorescens. Furthermore, KSL showed anticancer effects against five carcinoma cell lines, HT29, Hela, MCF7, SK-LU-1 and AGS, in a dose-dependent manner with the I C50 values of 0.80–1.94 µM, whereas its inhibition activities on cancer cells were not detected in the presence of yeast mannan, an inhibitor against lectin KSL. The cultivated red alga K. striatus could also be a good source of functional lectin(s) for application as anticancer agents. Graphic abstract
Keywords Anticancer activity · Kappaphycus striatus · Lectin · Molecular mass · Primary structure
Extended author information available on the last page of the article
13
Vol.:(0123456789)
Journal of Natural Medicines
Introduction Lectins, carbohydrate-binding proteins, are present in various organisms ranging from viruses to humans, and play important roles as recognition molecules in cell–cell or cell–matrix interactions. Due to the ability to discriminate differences in carbohydrate structures, not only lectins are available as potential reagents in many research fields but they are promising candidates for medicinal and clinical application [1]. Marine algae are a good source of novel lectins. Algal lectins possess unique molecular structures and carbohydrate-binding specificities distinct from known lectins from other sources, which make them useful for applications [2, 3]. Recently, some the high-mannose specific lectins from the eukaryotic marine red algae attracted some attention as potential sources of new lectins with antibacterial, antiviral (HIV, SARS-CoV and influenza virus) and anticancer activities [4–11]. Thus, marine algal lectins may become a novel source of antiviral and anticancer compounds for biochemical and medicinal app
Data Loading...
