Preparation and Characterization of Chitosan/Silver Nano-composite and its Application on Nile Water as Antibacterial Ma
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MRS Advances © 2020 Materials Research Society DOI: 10.1557/adv.2020.110
Preparation and Characterization of Chitosan/Silver Nano-composite and its Application on Nile Water as Antibacterial Materials Heba Elfaig1; Wala Elsayed2; and Hasabo Ahmed1 1
Sudan University of science and technology, Sudan.
2
University of Khartoum, Sudan.
Abstract:
The prepared silver/chitosan nanocomposite and chitosan nanoparticles in this study may demonstrate the potential in optimizing the minimum amount required to achieve complete inactivation of various Coliform bacteria in Nile water. Chitosan nanoparticles were prepared based on the ionic gelation of the prepared chitosan and silver nanoparticles were reduced by Solenostemma Argel extract. Finally, chitosan silver-loaded nanoparticles were prepared by dispersing silver nanoparticles onto the chitosan nanoparticles. The SEM images exhibited a diameter range of 10 nm–30 nm for both of the fabricated silver nanoparticles and chitosan nanoparticles. The UV-Vis analysis confirmed the formation of Ag nanoparticles by the appearance of the characteristic peak at 410 nm. The antibacterial activity of chitosan nanoparticles and silver-loaded nanoparticles was evaluated against the Coliform bacteria. Results show an improvement in the inhibition of the growth of various bacteria tested when silver nanoparticles were introduced which was (0.03g/100ml). Consequently, chitosan silverloaded nanoparticles could be recommended as an efficient antibacterial material for water disinfection.
1. Introduction Chitosan and its derivatives have attracted attention due to their miscellaneous applications and their antimicrobial, biodegradability and antifungal activity. This nontoxic abundant polymer is extracted from chitin, which is produced naturally by crustacean, like crawfish, shrimps, crabs, and lobsters. the extraction process includes a
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sequential chemical process involving demineralization, deproteinization, decolorization, and deacetylation.[1]. Chitosan nanoparticles (NPs) have demonstrated improved biological properties such as biocompatibility, antifungal, and antibacterial activity than chitosan. They are used in a variety of different products and applications, ranging from pharmaceutical, drug delivery, tissue engineering, and food packaging to bio-sensing, enzymes immobilization, fuel cell manufacturing, and water treatment. They can be synthesized by employing one of the following methods: polyelectrolyte complex, reverse micellar, microemulsion, and Ionic gelation method. Ionic gelation method is a simple and mild method which is widely used for the preparation of chitosan NPs[2]. Chitosan-silver nanocomposite was fabricated using a three-step method which includes preparation of silver ion-poly(ethylene glycol), the addition of chitosan mat
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