Biogenic preparation and characterization of Pyropia yezoensis silver nanoparticles ( P.y AgNPs) and their antibacterial
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RESEARCH PAPER
Biogenic preparation and characterization of Pyropia yezoensis silver nanoparticles (P.y AgNPs) and their antibacterial activity against Pseudomonas aeruginosa Selvakumari Ulagesan1 · Taek‑Jeong Nam1 · Youn‑Hee Choi1,2 Received: 28 July 2020 / Accepted: 23 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Marine algae play key roles in several medical, pharmaceutical, agricultural, and aquacultural applications. Furthermore, biosynthesized nanomaterials are becoming an alternative to conventional antibiotics in cost-effective, biocompatible, and non-toxic treatments for bacterial infections. This study features biogenic synthesis of silver nanoparticles using an aqueous extract of the marine red algae Pyropia yezoensis. The formation of silver nanoparticles was initially confirmed by UV–Vis spectroscopy and FTIR spectra were used to identify functional groups. The average crystalline size of the silver nanoparticles was around 20–22 nm, as determined by XRD analysis. Particle size was confirmed by SEM and TEM analyses, which also showed spherical particles without agglomeration. The antibacterial properties of the nanoparticles were assessed against both Gram-positive and Gram-negative bacterial cultures with significant activity observed against Gram negative P. aeruginosa. Our Pyropia yezoensis silver nanoparticles (P.y AgNPs) reduced the growth of P. aeruginosa at concentrations of 200 and 400 µg/ml. Our results strongly imply that P.y AgNPs may be useful in treating bacterial infections. Keywords Pyropia yezoensis · Marine algae · Silver nanoparticles · Pseudomonas aeruginosa · Antimicrobial activity · Natural product
Introduction Bacterial outbreaks, originating from human contact, food poisoning, contaminated water reservoirs, and bioterrorism remain a major global health threat [1, 2]. In addition, several antibiotics are being used indiscreetly for controlling disease-causing microbes, resulting in the emergence of pathogenic bacterial strains with acquired resistance to nearly all current antibiotics including penicillin, streptomycin, tetracycline, and vancomycin [3]. Pseudomonas aeruginosa and Staphylococcus aureus are notorious pathogen * Taek‑Jeong Nam [email protected] * Youn‑Hee Choi [email protected] 1
Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Republic of Korea
Department of Marine Bio‑Materials and Aquaculture, Pukyong National University, 45, Yongso‑ro, Nam‑Gu, Busan 48513, Republic of Korea
2
which can cause infections in the blood, lungs (pneumonia), or other parts of the body after surgery they are more virulent and resistant to presently available antibiotics [4]. Therefore, new antibacterial agents are needed to treat these antibiotic-resistant bacterial infections. In 2017, the World Health Organization (WHO) published a list of antibioticresistant pathogens that pose a risk to human health (WHO, 2017). This report clearly suggested that, given the seriousness of drug-resistant bacteria, developm
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