Production of an epigenetic mutant population of Populus nigra : DNA methylation and phenotype analyses
- PDF / 797,435 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 17 Downloads / 189 Views
ORIGINAL ARTICLE
Production of an epigenetic mutant population of Populus nigra: DNA methylation and phenotype analyses Shanchen Zhong1 • Yanan Gao1 • Junqian Lu1 • Changjun Ding1 • Xiaohua Su1 • Bingyu Zhang1 Received: 5 May 2020 / Accepted: 1 September 2020 Ó Society for Plant Biochemistry and Biotechnology 2020
Abstract 5-Azacytidine (5-AzaC) causes hypomethylation of genomic DNA and induces phenotype variation in many plant species. Modulating the methylation status of cytosine by 5-AzaC to generate novel phenotypes in poplars have not been attempted. In this study, a population of 288 plants was regenerated from leaves of Populus nigra on medium supplemented with 100–1000 lM 5-AzaC. The differentiation of leaves was delayed and the differentiation rate decreased as the 5-AzaC concentration increased. Compared with plants regenerated on 5-AzaC-free medium (control), the plants regenerated on high-5-AzaC medium (600–1000 lM) had significantly higher DNA methylation levels and were shorter, whereas the plants regenerated on low-5-AzaC medium were unchanged; the plants regenerated on 100 lM 5-AzaC medium had significant more leaves; the plants regenerated on 400 lM 5-AzaC medium had significantly higher leaf chlorophyll b and total chlorophyll contents, while the plants on 800 and 1000 lM 5-AzaC medium had a significantly lower chlorophyll a content; most photosynthetic parameters of the plants regenerated on 5-AzaC-treated medium decreased, except for the net photosynthetic rate (Pn) and transpiration rate (Tr) of plants on 100, 200, and 400 lM medium, and the instant water utilization efficiency (WUEi) of plants on 1000 lM medium. The superoxide dismutase activity of plants on C 400 lM 5-AzaC medium was significantly higher than in the control. Significant weak correlations were found between the methylation level of regenerants and plant height, chlorophyll a content, Pn, Tr, stomatal conductance, intercellular CO2 concentration, and WUEi, indicating these phenotypic changes were related to the methylation changes. Our results demonstrated the use of 5-AzaC at the regeneration stage induced epigenetic as well as phynotypic variations in poplar regenerants and generated plant materials for genetic breeding and epigenetic studies. Keywords Populus nigra 5-AzaC Plant regeneration DNA methylation MSAP Phenotypic variation Abbreviations 5-AzaC 5-Azacytidine IBA Indole-3-butyric acid Ci Intercellular CO2 concentration MSAP Methyl-sensitive amplification polymorphism MS Murashige and Skoog medium NAA Naphthalene acetic acid Pn Net photosynthetic rate
& Xiaohua Su [email protected] Bingyu Zhang [email protected] 1
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, 100091 Beijing, China
Gs SOD Tr WUEi
Stomatal conductance Superoxide dismutase Transpiration rate Water utilization efficiency
Introduction The European black poplar (Populus nigra L.) is a
Data Loading...
