Production of pyruvate from Ulva reticulata using the alkaliphilic, halophilic bacterium Halomonas sp. BL6
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23RD INTERNATIONAL SEAWEED SYMPOSIUM, JEJU
Production of pyruvate from Ulva reticulata using the alkaliphilic, halophilic bacterium Halomonas sp. BL6 Hoang Thi Lan Anh 1 & Yoshikazu Kawata 2 & Luu Thi Tam 1 & Le Thi Thom 1 & Nguyen Cam Ha 1 & Hoang Thi Minh Hien 1 & Ngo Thi Hoai Thu 1 & Pham Quang Huy 3 & Dang Diem Hong 1,4,5 Received: 24 July 2019 / Revised and accepted: 1 January 2020 # Springer Nature B.V. 2020
Abstract Green macroalgae have gained attention as promising renewable sources for biorefining. Despite the wide potential availability of green macroalgae, their utilization has been limited to ethanol production, hindering their further application. In this study, we report that a bacterium, Halomonas sp. strain BL6 (isolated from a mangrove forest in Bach Long, Nam Dinh Province, Vietnam), produces pyruvate from a saccharified solution of the green seaweed Ulva reticulata and secretes it into the medium. Pyruvate, an important α-oxocarboxylic acid, plays a central role in energy and carbon metabolism in living organisms and is used mainly for the synthesis of various chemicals and polymers or as an ingredient or additive in food, cosmetics, and pharmaceuticals. To investigate the possibility of using U. reticulata from the seashore of Vietnam as biomass feedstock, the chemical composition and saccharification yield of this seaweed were studied. Dry biomass of U. reticulata was found to contain 65.5% carbohydrate, 10.3% protein, 1.8% lipid, and 10.6% ash. Reducing sugar content reached 608.79 mg g−1 of biomass after pretreatment with diluted acid and 24 h of incubation with 50 IU g−1 Viscozyme L. The resulting sugars were fermented by Halomonas sp. strain BL6 to produce pyruvate, and the maximal pyruvate concentration reached 55.23 g L−1 after 72 h of cultivation. This study is the first to report the production of valuable compounds other than bioethanol products, such as pyruvate, from U. reticulata hydrolysate by a Halomonas strain. Keywords Halomonas . Green seaweed . Halophilic bacteria . Pyruvate . Ulva reticulata
Introduction Recently, macroalgae have received much attention as possible renewable sources for biofuels and bioproducts (Jang et al. * Yoshikazu Kawata [email protected] * Dang Diem Hong [email protected] 1
Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
2
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Osaka, Japan
3
School of Applied Biosciences, Kyungpook National University, Daegu, South Korea
4
Thuyloi University, Hanoi, Vietnam
5
Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
2012). Macroalgae (also called seaweed) is a collective term referring to a series of non-phylogenetic, multicellular, macroscopic and eukaryotic organisms (Jiang et al. 2016). Normally, they are classified into three groups of algae, green (Chlorophyceae), red (Rhodophyceae), or brown (Phaeophyceae
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