Noviherbaspirillum galbum sp. nov., a bacterium isolated from soil
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ORIGINAL PAPER
Noviherbaspirillum galbum sp. nov., a bacterium isolated from soil Yuna Park1 · Soohyun Maeng1 · Sang Eun Lee1 · Joo Hyun Han1 · Young Koung Lee2 · Jaesung Oh2 · Baigalmaa Jigden3 · Ju Hyeon Kang1 · Myung Kyum Kim1 Received: 29 March 2020 / Revised: 21 September 2020 / Accepted: 1 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A Gram-stain-negative, aerobic, non-motile and yellow-colored bacterium, strain 17J57-3 T, was isolated from soil collected in Pyeongchang city, Korea. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 17J57-3 T formed a distinct lineage within the family Oxalobacteraceae (order Burkholderiales, class Betaproteobacteria). Strain 17J57-3 T was the most closely related to Noviherbaspirillum humi U15T (96.4% 16S rRNA gene sequence similarity) and Noviherbaspirillum massiliense JC206T (96.2%). The draft genome size of strain 17J57-3 T was 6,117,206 bp. Optimal growth occurred at 30 °C, pH 7.0 without NaCl. The predominant cellular fatty acids were summed feature 3 ( C16:1 ω6c/C16:1 ω7c) and C16:0. The major respiratory quinone was Q-8. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Biochemical, chemotaxonomic and phylogenetic analyses indicated that strain 17J57-3 T represents a novel bacterial species within the genus Noviherbaspirillum, for which the name Noviherbaspirillum galbum is proposed. The type strain of Noviherbaspirillum galbum is 17J57-3 T (= KCTC 62213 T = NBRC 114384 T). Keywords Novel species · Noviherbaspirillum · Oxalobacteraceae · Taxonomy
Introduction The genus Noviherbaspirillum is a member of the family Oxalobacteraceae in the class Betaproteobacteria. Genus Noviherbaspirillum was first reported with Noviherbaspirillum malthae as type species and reclassification of several Herbaspirillum species (N. aurantiacum, N. canariense, N. psychrotolerans and N. soli) as Noviherbaspirillum members Communicated by Erko stackebrandt.
(Lin et al. 2013). At the time of writing the manuscript, the genus Noviherbaspirillum consists of 12 species (www.bacte rio.net/noviherbaspirillum.html). Members of the genus Noviherbaspirillum have been isolated from various environments such as oil-contaminated site, grassland and old volcanic mountain (Carro et al. 2012; Chaudhary and Kim 2017; Lin et al. 2013). In this study, we report polyphasic taxonomic analysis of a novel strain 17J57-3 T isolated from soil sampled in Pyeongchang county, Korea and propose the name Noviherbaspirillum galbum sp. nov.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02076-7) contains supplementary material, which is available to authorized users.
Materials and methods
* Myung Kyum Kim [email protected]
Organism and culture conditions
1
Department of Bio and Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul 01797, Republic of Korea
2
Plasma Technology Research Cen
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