Production Process of Bioethanol Fuel Using Supported Saccharomyces Cerevisiae Cells

A new immobilization process of Saccharomyces cerevisiae cells with calcium alginate and coating with solubilized chitosan in citric acid solution was developed. The biocatalyst is used in fermentation processes to produce bioethanol fuel through the use

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Abstract A new immobilization process of Saccharomyces cerevisiae cells with calcium alginate and coating with solubilized chitosan in citric acid solution was developed. The biocatalyst is used in fermentation processes to produce bioethanol fuel through the use of carbon sources such as glucose, sugarcane juice, and corn hydrolysate, which provided very good yields of productivity (71.3–88.7%) and shows excellent biocatalyst stability during all production processes. Keywords Bioethanol fuel · Calcium alginate beads · Fermentation process

Author Contributions: Fardelone, L. C. and Bella de Jesus, T. S. contributed equally L. C. Fardelone (B) · T. S. B. de Jesus · G. P. Valença · J. R. Nunhez School of Chemical Engineering, University of Campinas - Unicamp, Campinas, Brazil e-mail: [email protected] T. S. B. de Jesus e-mail: [email protected] G. P. Valença e-mail: [email protected] J. R. Nunhez e-mail: [email protected] J. A. R. Rodrigues · P. J. S. Moran Chemistry Institute, University of Campinas - Unicamp, Campinas, Brazil e-mail: [email protected] P. J. S. Moran e-mail: [email protected] © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2021 Y. Iano et al. (eds.), Proceedings of the 5th Brazilian Technology Symposium, Smart Innovation, Systems and Technologies 202, https://doi.org/10.1007/978-3-030-57566-3_33

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1 Introduction The research for more efficient biofuel processes is strategic for Brazil, due to climate change, world economic policies and the growing demand for sustainability regarding the issue of energy generation [1–6]. Thus, bioethanol fuel has gained worldwide prominence because it is biodegradable, has clean combustion due to the presence of oxygen in its composition, and reduces the emission of nitrogen oxides and particulate matter, as well as reducing CO2 and SO2 emissions into the atmosphere [5]. The process of alcoholic fermentation using sugarcane juice S. cerevisiae cells is a process well established in the Brazilian mills [1, 4], however, the search for cost reduction, unit operations and process time, as well as productivity increase is a big challenge, which motivates us in this development of bioethanol fuel production. Also, the production of bioethanol fuel from corn has numerous advantages, such as logistics and storage, shorter plant cultivation cycle time and depending on the variety 3–4 annual harvests, can also be obtained during the sugar cane off-season, and if planted in the same area can bring improvements to the soil, decreased fertilization and correction of it [7, 8]. Corn also has lower production costs and its planting is established in different regions of the country [8]. Our research group has been working on fermentative processes for bioethanol fuel production using cellular supports such as calcium alginate [9], Montmorillonite K-10 [10], and other clays. The literature also describes supported processes that can promote longer process tim