Bioethanol Production from Azolla filiculoides by Saccharomyces cerevisiae , Pichia stipitis , Candida lusitaniae , and
- PDF / 1,177,622 Bytes
- 13 Pages / 439.37 x 666.142 pts Page_size
- 76 Downloads / 211 Views
Bioethanol Production from Azolla filiculoides by Saccharomyces cerevisiae, Pichia stipitis, Candida lusitaniae, and Kluyveromyces marxianus Mariam H. Chupaza 1,2,3 & Yu-Rim Park 1 & So Hee Kim 1 & Ji Won Yang 1 & Gwi-Teak Jeong 1 & Sung-Koo Kim 1 Received: 30 July 2020 / Accepted: 29 September 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
Ethanol was produced by separate hydrolysis and fermentation using Azolla filiculoides as a biomass. Thermal acid hydrolysis and enzymatic saccharification were used as pretreatment methods to produce monosaccharides from Azolla. The optimal content for thermal acid hydrolysis of 14% (w/v) Azolla weed slurry produced 16.7-g/L monosaccharides by using 200 mM H2SO4 at 121 °C for 60 min. Enzymatic saccharification using 16 U/mL Viscozyme produced 61.6 g/L monosaccharide at 48 h. Ethanol productions with ethanol yield coefficients from Azolla weed hydrolysate using Kluyveromyces marxianus, Candida lusitaniae Saccharomyces cerevisiae, and Pichia stipitis were 26.8 g/L (YEtOH = 0.43), 23.2 g/L (YEtOH = 0.37), 18.2 g/L (YEtOH = 0.29), and 13.7 g/ L (YEtOH = 0.22), respectively. Saccharomyces cerevisiae produces the lowest yield as it utilized only glucose. Bioethanol from Azolla weed hydrolysate can be successfully produced by using Kluyveromyces marxianus because it consumed the mixture of glucose and xylose completely within 60 h. Keywords Bioethanol . Azolla filiculoides . Kluyveromyces marxianus . Candida lusitaniae . Pichia stipitis . Saccharomyces cerevisiae
Mariam H. Chupaza and Yu-Rim Park are co-first authors.
* Sung-Koo Kim [email protected]
1
School of Marine Fisheries, and Life Science (Major in Biotechnology), Pukyong National University, 48513 Busan, Republic of Korea
2
KOICA-PKNU International Graduate Program of Fisheries Science, Pukyong National University, Busan 48513, Republic of Korea
3
Department of Fishing and Fish Processing, Fisheries Education and Training Agency, P.O. Box 83, Bagamoyo, Costal Region, Tanzania
Applied Biochemistry and Biotechnology
Introduction In the twenty-first century, the society is facing global challenges on how and where to get sufficient and sustainable energy for transportation, heating and industrial processes, and raw material for different industries to run the world in a sustainable way [1]. Currently, the goals of study are to reduce our dependency on fossil fuels and prevent further deforestation and competition with foods. This has triggered an extensive search for domestication of new bioenergy feedstock which can generate substantial renewable biomass over a short period with rich bioenergy molecules which can be converted into biofuels using a set of well-established technologies [2]. Since fossil energy are limited and their large-scale use as fuels has a negative impact on global climate, thus fuel alternative resources for energy productions are required [3]. Zhang et al. [4] have shown that renewable energy is recognized as the next generation that will replace human depe
Data Loading...
