Proliferation and Adhesion of L929 Fibroblasts on Surface with Different Microtopography
- PDF / 6,181,482 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 44 Downloads / 171 Views
Proliferation and Adhesion of L929 Fibroblasts on Surface with Different Microtopography Yuta Kurashina1, Shogo Miyata1, Jun Komotori1 and Tadayoshi Koyama2 1 Faculty of Science and Technology, Keio University, Yokohama 223-0061, Japan 2 Medicalscience Co. Ltd., Yokohama 231-0033, Japan
ABSTRACT Three types of 316L stainless steel surface with different topography were prepared by a Fine Particle Peening (FPP) treatment using titania, silica and alumina shot particles and analyzed the cell proliferation and cell-scaffold interaction. FPP-treated surface with titania and silica particles had micro asperities at low frequency. On the other hand, the alumina treated surface had micro asperities at high frequency. L929 fibroblasts were seeded on these specimens and then the number of cells was counted after 72 hours of culturing. The FPP-treated surfaces showed good cell proliferation comparing to polished surface. This indicates that micro asperities formed on the surface encourage cell adhesion. Cell adhesion behavior was evaluated by a scanning electron microscope (SEM) and a fluorescence microscope. Dense filopodia were observed when cells cultured on the FPP-treated surface. This means that FPP treatment enhances cell adhesion and proliferation. The number of cells observed on the FPP-treated surface depended on the shape of asperities formed by FPP treatment; the highest cell counts were obtained on alumina treated surface. This is because cell migration was not inhibited by the shape of alumina treated surface asperities. INTRODUCTION Cell–matrix interactions are dependent on the nature of the extra-cellular matrix (ECM). The ECM is crucial in mediating cell adhesion to scaffolds, the proliferation of cells, the production of proteins, and the degree of cell attachment to materials [1]. Currently, treated surfaces, such as collagen, are being used widely as cell culture scaffolds [2]. One key concern regarding scaffold surfaces is their topographical features, since surface topography stimulates changes in proliferation [3] and differentiation [4], as well as adhesion to substrates [5]. A variety of techniques have been reported for creating substrates using different materials that exhibit controlled topography with low and high frequency spacing. The positive effects of topography on fibroblast proliferation and, more generally, the effect of substrate topography on cell behavior, remain poorly understood. Moreover, the results presented in the preference for cell adhesion on randomly rough surfaces remains controversial, and to date no study has been published interpreting or even summarizing these results. In this work, both cell adhesion and cell migration experimental approaches were used to understand the role microtopographies play in mediating cell proliferation. In these experiments, fibroblast proliferation in response to Fine Particle Peening (FPP) treated metal surfaces was investigated using cell filopodia extensions and cell migration.
EXPERIIMENT FPP treattment techniiques used too modify surrf
Data Loading...
