Protein Engineering Techniques Gateways to Synthetic Protein Univers
This brief provides a broad overview of protein-engineering research, offering a glimpse of the most common experimental methods. It also presents various computational programs with applications that are widely used in directed evolution, computational a
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Krishna Mohan Poluri Khushboo Gulati
Protein Engineering Techniques Gateways to Synthetic Protein Universe
SpringerBriefs in Applied Sciences and Technology Forensic and Medical Bioinformatics
Series editors Amit Kumar, Hyderabad, India Allam Appa Rao, Hyderabad, India
More information about this series at http://www.springer.com/series/11910
Krishna Mohan Poluri Khushboo Gulati •
Protein Engineering Techniques Gateways to Synthetic Protein Universe
123
Krishna Mohan Poluri Department of Biotechnology and Center for Nanotechnology Indian Institute of Technology Roorkee Roorkee, Uttarakhand India
Khushboo Gulati Department of Biotechnology Indian Institute of Technology Roorkee Roorkee, Uttarakhand India
ISSN 2191-530X ISSN 2191-5318 (electronic) SpringerBriefs in Applied Sciences and Technology ISSN 2196-8845 ISSN 2196-8853 (electronic) Springer Briefs in Forensic and Medical Bioinformatics ISBN 978-981-10-2731-4 ISBN 978-981-10-2732-1 (eBook) DOI 10.1007/978-981-10-2732-1 Library of Congress Control Number: 2016954620 © The Author(s) 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Springer imprint is published by Springer Nature The registered company is Springer Nature Singapore Pte Ltd. The registered company address is: 152 Beach Road, #22-06/08 Gateway East, Singapore 189721, Singapore
Dedicated to all our teachers and mentors for their invaluable guidance and unconditional support throughout our research careers Krishna Mohan Poluri Khushboo Gulati
Preface
Proteins are the string of amino acids that play key roles in almost all the physiological and pathological events that are occurring in the body. Functionality of proteins is directly related to the structure they attain, which ultimately depends on the composition of their unique amino acid sequence. Protein engineering is a fledging field and a creative process to design the target proteins or signaling networks with desirable structure and functions. The idea of p
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