Protein Engineering Methods and Protocols
This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational
- PDF / 10,584,143 Bytes
- 347 Pages / 504.63 x 737.01 pts Page_size
- 42 Downloads / 198 Views
Uwe T. Bornscheuer Matthias Höhne Editors
Protein Engineering Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Protein Engineering Methods and Protocols
Edited by
Uwe T. Bornscheuer Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Greifswald, Germany
Matthias Höhne Protein Biochemistry, Institute of Biochemistry, Greifswald University, Greifswald, Germany
Editors Uwe T. Bornscheuer Department of Biotechnology and Enzyme Catalysis Institute of Biochemistry Greifswald University Greifswald, Germany
Matthias Ho¨hne Protein Biochemistry Institute of Biochemistry Greifswald University Greifswald, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7364-4 ISBN 978-1-4939-7366-8 (eBook) DOI 10.1007/978-1-4939-7366-8 Library of Congress Control Number: 2017953803 © Springer Science+Business Media LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Since the discovery of proteins and their numerous roles in life, scientists are fascinated to study the molecular basis of how proteins function. It is amazing to see the plethora of protein structures and mechanisms that appeared during evolution, and the creativity, which is operating in nature’s continuing process of tailoring and fine-tuning proteins and, thus, life itself. Proteins, especially enzymes, are also the key players of biocatalysis and biotechnology and thus they are linked
Data Loading...
