Synthetic Protein Switches Methods and Protocols
This detailed book provides state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Beginning with general strategies, the volume continues with coverage of
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Viktor Stein Editor
Synthetic Protein Switches Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Synthetic Protein Switches Methods and Protocols
Edited by
Viktor Stein Fachbereich Biologie, Technische Universität Darmstadt, Darmstadt, Germany
Editor Viktor Stein Fachbereich Biologie Technische Universität Darmstadt Darmstadt, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6938-8 ISBN 978-1-4939-6940-1 (eBook) DOI 10.1007/978-1-4939-6940-1 Library of Congress Control Number: 2017933284 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Synthetic protein switches with custom response functions have become invaluable tools in basic research and biotechnology for monitoring biomolecular analytes or actuating cellular functions in a rapid, specific, integrated, and autonomous fashion. This book provides a comprehensive summary of state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Protocols are applicable to life scientists from diverse research fields that range from traditional, discovery-centered disciplines such as cancer research to newly emerging disciplines such as synthetic biology. Chapters are grouped into separate sections focusing on different types of switches, sensors, and actuators. Starting with a general view, I first d
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