Protein NMR Techniques

The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon t

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MOLECULAR BIOLOGY™

Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Protein NMR Techniques Third Edition Edited by

Alexander Shekhtman and David S. Burz Department of Chemistry, University at Albany, State University of New York, Albany, NY, USA

Editors Alexander Shekhtman Department of Chemistry University at Albany State University of New York 1400 Washington Avenue Albany, NY 12222, USA [email protected]

David S. Burz Department of Chemistry University at Albany State University of New York 1400 Washington Avenue Albany, NY 12222, USA [email protected]

ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-479-7 e-ISBN 978-1-61779-480-3 DOI 10.1007/978-1-61779-480-3 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011943883 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)

Preface The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology, and cell biology research that were impossible to study as recently as 10 years ago. The potential to study macromolecular systems that were once considered too large or too transient or too complex by using NMR spectroscopy is now being realized with the development of innovative technologies. Standard NMR technologies are also getting a facelift in part due to the pervasive nature of high-throughput approaches in biochemical and biomedical research. These advances warrant a new edition of Protein NMR Techniques that includes an authoritative but down-to-earth description of new methodologies. This edition consists of 24 chapters divided into four major categories: NMR sample preparation, solution NMR methodologies, solid-state NMR methodologies, and data processing. The material presented contains enough detail for use not only in specialized NMR laboratories, but in biochemical, molecular, and cell biology research labs that have access to high-field NMR spectrometers. Preparing proteins for NMR spectroscopy can be a time-consuming process that may take longer than data collection and analysis combined. Expression in bacterial ce