Proteomic analysis of blastema formation in regenerating axolotl limbs
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BioMed Central
Open Access
Research article
Proteomic analysis of blastema formation in regenerating axolotl limbs Nandini Rao1, Deepali Jhamb2, Derek J Milner3, Bingbing Li1, Fengyu Song4, Mu Wang5, S Randal Voss6, Mathew Palakal2, Michael W King5, Behnaz Saranjami1, Holly LD Nye3, Jo Ann Cameron3 and David L Stocum*1 Address: 1Department of Biology and Center for Regenerative Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN, USA, 2School of Informatics and Center for Regenerative Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN, USA, 3Department of Cell and Developmental Biology, and Regeneration Biology and Tissue Engineering Theme, Institute for Genomic Biology, University of Illinois-Urbana Champaign, Urbana, IL, USA, 4Department of Oral Biology, School of Dentistry and Center for Regenerative Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN, USA, 5Department of Biochemistry, School of Medicine and Center for Regenerative Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN, USA and 6Department of Biology and Spinal Cord and Brain Injury Center, University of Kentucky at Lexington, Lexington, KY, USA Email: Nandini Rao - [email protected]; Deepali Jhamb - [email protected]; Derek J Milner - [email protected]; Bingbing Li - [email protected]; Fengyu Song - [email protected]; Mu Wang - [email protected]; S Randal Voss - [email protected]; Mathew Palakal - [email protected]; Michael W King - [email protected]; Behnaz Saranjami - [email protected]; Holly LD Nye - [email protected]; Jo Ann Cameron - [email protected]; David L Stocum* - [email protected] * Corresponding author
Published: 30 November 2009 BMC Biology 2009, 7:83
doi:10.1186/1741-7007-7-83
Received: 2 September 2009 Accepted: 30 November 2009
This article is available from: http://www.biomedcentral.com/1741-7007/7/83 © 2009 Rao et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Following amputation, urodele salamander limbs reprogram somatic cells to form a blastema that selforganizes into the missing limb parts to restore the structure and function of the limb. To help understand the molecular basis of blastema formation, we used quantitative label-free liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)-based methods to analyze changes in the proteome that occurred 1, 4 and 7 days post amputation (dpa) through the mid-tibia/fibula of axolotl hind limbs. Results: We identified 309 unique proteins with significant fold change relative to controls (0 dpa), representing 10 biological process categories: (1) signaling, (2) Ca2+ binding and translocation, (3) transcription, (4) translation, (5)
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