Proteomic and metabolic characterization of membrane vesicles derived from Streptococcus mutans at different pH values
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GENOMICS, TRANSCRIPTOMICS, PROTEOMICS
Proteomic and metabolic characterization of membrane vesicles derived from Streptococcus mutans at different pH values Yina Cao 1,2 & Yan Zhou 1,2 & Dongru Chen 1,2 & Ruixue Wu 1,2 & Lihong Guo 2,3 & Huancai Lin 1,2 Received: 19 December 2019 / Revised: 12 March 2020 / Accepted: 20 March 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Bacterial membrane vesicles (MVs) are used as a tool for intercellular communication and seem essential for bacterial survival. However, few data are available on MVs generated by Streptococcus mutans, which is the main aetiological agent of dental caries. The present study presents an integrated proteomics and metabolomics analysis of MVs isolated from S. mutans at initial pH values of 7.5 and 5.5 and explores their function. The results showed that S. mutans releases more MVs with smaller diameters under acidic conditions than under neutral conditions. Proteomic analysis showed 344 common vesicular proteins, including various virulence factors. The expressions of 140 individual proteins and 37 metabolites were altered as a result of culturing S. mutans at different pH values. Co-analyses of proteomic and metabolomics data indicated that ABC transporters underwent significant changes under acid pressure. We concluded that S. mutans produced MVs at different pH values to carry proteins associated with cariogenesis. Moreover, the alterations of S. mutans MVs under acid pressure were associated with ABC transporters. These results increase our knowledge of S. mutans MVs and imply that S. mutans MVs may play a functional role in carious infection. Key points • S. mutans MVs contained virulence factor-related proteins, even at low pH values. • Integrated proteomics and metabolomics analysis showed that S. mutans MVs alterations under acidic conditions were associated with ABC transporters. Keywords Streptococcus mutans . Membrane vesicles . Proteomic . Metabolomic . Biofilm formation
Introduction Dental caries is one of the most widespread chronic infectious diseases. According to the result of the 4th National Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00253-020-10563-6) contains supplementary material, which is available to authorized users. * Lihong Guo [email protected] * Huancai Lin [email protected] 1
Department of Preventive Dentistry, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China
2
Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China
3
Department of Endodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China
Oral Health Survey of China, the prevalence of dental caries among 3- to 5-year-olds was 62.5% (Du et al. 2018), and that among 12- to 15-year-olds was 41.9% (Quan et al. 2018). In the world, dental caries has historically been a major public health issue (Disease et al. 2017). Streptococcus mutans, which is a Gram-posi
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