Proteomics Advances in the Study of Leishmania Parasites and Leishmaniasis
Leishmania spp. are digenetic parasites which cause a broad spectrum of fatal diseases in humans. These parasites, as well as the other trypanosomatid, regulate gene expression at the post-transcriptional and post-translational levels, so that a poor corr
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Proteomics Advances in the Study of Leishmania Parasites and Leishmaniasis Jose Batista de Jesus, Camila Mesquita-Rodrigues, and Patricia Cuervo
Abstract Leishmania spp. are digenetic parasites which cause a broad spectrum of fatal diseases in humans. These parasites, as well as the other trypanosomatid, regulate gene expression at the post-transcriptional and post-translational levels, so that a poor correlation is observed between mRNA content and translated proteins. The completion of the genomic sequencing of several Leishmania species has enormous relevance to the study of the leishmaniasis pathogenesis. The combination of the available genomic resources of these parasites with powerful high-throughput proteomic analysis has shed light on various aspects of Leishmania biology as well as on the mechanisms underlying the disease. Diverse proteomic approaches have been used to describe and catalogue global protein profiles of Leishmania spp., reveal changes in protein expression during development, determine the subcellular localization of gene products, evaluate host-parasite interactions and elucidate drug resistance mechanisms. The characterization of these proteins has advanced, although many fundamental questions remain unanswered. Here, we present a historic review summarizing the different proteomic technologies applied to the study
J.B. de Jesus (*) Departamento de Engenharia de Biossistemas, Universidade Federal de São João Del Rei, São João Del Rei, MG, Brazil Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil e-mail: [email protected] C. Mesquita-Rodrigues Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil P. Cuervo Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil A.L.S. Santos et al. (eds.), Proteins and Proteomics of Leishmania and Trypanosoma, Subcellular Biochemistry 74, DOI 10.1007/978-94-007-7305-9_14, © Springer Science+Business Media Dordrecht 2014
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of Leishmania parasites during the last decades and we discuss the proteomic discoveries that have contributed to the understanding of Leishmania parasites biology and leishmaniasis.
Abbreviations 1DE 2D-DIGE 2DE 2D-LC-MS/MS
One dimensional electrophoresis Two dimensional differential gel electrophoresis Two dimensional electrophoresis Two dimensional liquid chromatography coupled with tandem mass spectrometry ATP Adenosine triphosphate BLAST Basic local alignment search tool DNA Deoxyribonucleic acid EDTA Ethylenediamine tetra acetic acid ESI Electrospray ionization ESI-MS Electrospray ionization mass spectrometry GFP Green fluorescent protein GO Gene ontology GP46 Glycoprotein 46 GP63 Glycoprotein 63 also called leishmanolysin and major surface peptidase GTP Guanosine triphosphate HSP60 Heat shock protein 60 HSP70 Heat shock protein 70 HSP90 Heat shock protein 90 HSP100 Heat shock protein 100 IEF Isoelectric focalization IgG Immunogl
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