Purification and characterization of antioxidant peptides from yak ( Bos grunniens ) bone hydrolysates and evaluation of
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ORIGINAL ARTICLE
Purification and characterization of antioxidant peptides from yak (Bos grunniens) bone hydrolysates and evaluation of cellular antioxidant activity Xiaohui Sun1 • Kai Wang1 • Song Gao1 • Hui Hong1 • Longteng Zhang1 Huaigao Liu3 • Ligeng Feng1 • Yongkang Luo2
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Revised: 10 September 2020 / Accepted: 17 September 2020 Ó Association of Food Scientists & Technologists (India) 2020
Abstract In this study, papain and alcalase were used to generate antioxidant peptides from yak bone protein. The antioxidant activities of hydrolysates in vitro were evaluated by 2,20 -azinobios-(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity, total reducing power, ferrous ion chelating ability and hydroxyl radical scavenging activity. The hydrolysates generated by alcalase possessed the best antioxidant activity among unhydrolyzed protein and samples treated by papain, but the antioxidant activity decreased after simulated gastrointestinal digestion in vitro. The products of simulated gastrointestinal digestion were separated by ultrafiltration and high performance liquid chromatography, and the amino acid sequences of peptides were identified by mass spectrometry. The digestion sites within peptides were predicted by a bioinformatics strategy, and ten peptides were selected for synthesis. Among 10 synthetic peptides, Gly-PheHyp-Gly-Ala-Asp-Gly-Val-Ala, Gly-Gly-Pro-Gln-Gly-ProArg and Gly-Ser-Gln-Gly-Ser-Gln-Gly-Pro-Ala possessed strong antioxidant activities, among which Gly-Phe-Hyp-GlyAla-Asp-Gly-Val-Ala had a significant cytoprotective effect in Caco-2 cells under oxidative stress induced by H2O2, which reduced the formation of reactive oxygen species and malondialdehyde, and improved the activity of antioxidant
& Yongkang Luo [email protected]; [email protected] 1
Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
2
National R&D Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang 330022, Jiangxi, China
3
Anhui Guotai Biotechnology Co., Ltd, Xuancheng, Anhui Province, China
enzymes in cells. These results showed that yak bone peptides exhibited strong antioxidant activity and have a potential value as a new type of natural antioxidant. Keywords Yak bone Peptides Antioxidant activities Cytoprotective effect
Introduction Reactive oxygen species (ROS), produced during cellular respiration in aerobic organisms, can lead to oxidative stress and damage in cell or tissues (Chalamaiah et al. 2012). Oxidative stress is related to many human diseases, such as cancer, diabetes, hypertension, and neurodegenerative diseases (Young and Woodside 2001). Therefore, it is very important to remove ROS to maintain human health. The exploration of safe and natural antioxidants has arouse great interest in recent years. After enzymatic hydrolysis, some of the released peptides exhibit excellent antioxidant activities in vitro (Kumar et al. 2012).
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