Optimization of Ultrafiltration Membrane Separation Technology and Characterization of Peptides from Bovine Bone Marrow
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Optimization of Ultrafiltration Membrane Separation Technology and Characterization of Peptides from Bovine Bone Marrow Parhat Rozi1 · Wugulnisa Mamattohti1 · Xiaojun Yang1 · Abulimiti Kelimu1 · Guzalnur Askar1 · Shengjun Ma1 · Nigary Yadikar1 Accepted: 23 September 2020 © Springer Nature B.V. 2020
Abstract Salt extraction and Ultrafiltration membrane (UFM) separation technology of bovine bone marrow protein (BBMP) were optimized, and the structures of proteins and peptides, as well as biological activities were evaluated. The maximum protein content and yield obtained were 57.40 mg/mL, 56.80% respectively by salt extraction under the following conditions: concentration 0.5 mol/L, extraction of 2 times per 3 h; temperature, 45 °C. Maximum membrane flux and protein content reached to 25.29 L/(m2 h), 76.9 mg/mL using UFMs method optimized by the response surface methodology (RSM) at 10 kDa membrane, operation pressure 0.20 MPa; concentration rate 5, and temperature 40 °C. Among them, the 3 kDa permeate part has the highest radical scavenging capacity against DPPH and hydroxyl free radicals (41 ± 0.20%, 53 ± 0.35%, respectively). These isolated parts were characterized by Fourier transform infrared spectrometry (FT-IR), scanning electron microscopy (SEM), atomic force microscope (AFM), sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), and Liquid chromatography-Mass spectrometer-Mass spectrometer (LC–MS/MS). The key functional groups, molecular weight, and mass data accordance with the UFM separation results. Therefore, the separation of protein and peptides with different molecular weights can be achieved by UFM. This study can provide technical support for the full utilization and scientific application of BBM resources, as well as the technical and theoretical foundation for the development of peptides for potential health foods or drugs supplementary materials. Keywords Bovine bone marrow · Peptide · Optimization · Ultrafiltration membrane · Antioxidant activity · LC–MS/MS
Introduction Domestic animals are mainly slaughtered for meat which forms the major product, while all other officials become by-products providing numerous bio-based applications with potential raw materials (Borrajo et al. 2019; Mechmeche et al. 2016). By-products constitute nearly 60–70% of the slaughtered carcass, of which nearly 40% forms edible and 20% inedible. Also, some by-products of domestic animal slaughter are considered edible in most of the developing countries, apart from being used as casings for sausages in the developed countries. Many edible meat by-products are notable sources of essential nutrients (Michel et al. 1997; * Parhat Rozi [email protected] 1
College of Food Science and Pharmacy, Xinjiang Agricultural University, Nongda East Road 311, Urumqi 830052, Xinjiang, People’s Republic of China
Yao et al. 2020). On average, proteins associated with the meat industry by-products constitute more than one-eighth of total protein in the lean meat (Bhaskar et al. 2007). China has increasingly large
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