Separation and Purification of Antioxidant Peptides from Enzymatically Prepared Scorpion ( Buthus martensii Karsch) Prot
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Separation and Purification of Antioxidant Peptides from Enzymatically Prepared Scorpion (Buthus martensii Karsch) Protein Hydrolysates Ahmidin Wali1,2 · Atikan Wubulikasimu1,2 · Gao yanhua1 · Adil Omar1,2 · Amina Arken1,2 · Abulimiti Yili1 · Haji Akber Aisa1 Accepted: 6 November 2019 © Springer Nature B.V. 2019
Abstract The purpose of this study was to separate and purify antioxidant peptides from the scorpion (Buthus martensii Karsch) protein hydrolysates (SPHs). Scorpion protein (SP) was first hydrolyzed by trypsin, papain, and alcalase, respectively. Results from hydrolysis tests revealed that peptides hydrolyzed with papain showed the highest degree of hydrolysis (DH), yield and antioxidant activity. The effect of papain hydrolysis on scorpion protein was optimized using the response surface methodology. The highest DH (31.31%) and yield (52.02%) of SPHs were obtained under the following conditions: hydrolysis time, 4.0 h; hydrolysis temperature, 50 °C; and enzyme/substrate ratio, 2.43%. Ultrafiltration, gel filtration and reversed-phase high-performance liquid chromatography was used and two novel antioxidant peptides were obtained. The sequences of the peptides determined by MALDI–TOF–MS/MS were LPTETLH (MW: 810.43 Da, P4-1) and IEEDLER (MW: 903.44 Da, P4-2), respectively. The results revealed SPHs as a potential valuable bioresource for production of antioxidant peptides in the food system. Keywords Scorpion (Buthus martensii Karsch) protein · Antioxidant peptides · Antioxidant activity · Response surface methodology · Hydrolysis · Purification
Introduction Free radicals can be defined as molecular or molecular fragments containing one or more unpaired electrons in molecular orbitals, including active oxygen species (ROS) and reactive nitrogen species (RNS) (Chao-Zhi et al. 2013; Zhang et al. 2019). Currently, a plethora of studies have identified and characterized protein hydrolysates and antioxidant peptides from dietary sources by enzymatic hydrolysis, including those from chicken (Yangying et al. 2012), nuts (Yang
* Abulimiti Yili [email protected] 1
Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Academy of Sciences of the People’s Republic of China, Beijing South Road 40‑1, Urumqi 830011, Xinjiang, People’s Republic of China
University of Chinese Academy of Science, Beijing 100039, People’s Republic of China
2
et al. 2017), fish (Pan et al. 2016), egg white (Fan et al. 2019), and cereal (Agrawal et al. 2019). Scorpions are edible and have been used for treatment and health care in China and other Asian countries for thousands of years, and large-scale commercial farming has achieved good social and economic benefits (Guo et al. 2013; He et al. 2013; Yao et al. 2014). Scorpion has pharmacological effects of anti-thrombosis, anticoagulant, fibrinolysis, analgesic, anti-tumor, and anti-epileptic. (Shi et al. 2015). With the emergence of large number of new methods for the study and identification of scorpion body parts
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