An evaluation of the in vitro antioxidant and antidiabetic potentials of camel and donkey milk peptides released from ca
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ORIGINAL ARTICLE
An evaluation of the in vitro antioxidant and antidiabetic potentials of camel and donkey milk peptides released from casein and whey proteins Ecem Akan1
Revised: 23 September 2020 / Accepted: 1 October 2020 Ó Association of Food Scientists & Technologists (India) 2020
Abstract In this study, some biological activities including antioxidant activity (DPPH radical scavenging activity, ABTS radical scavenging activity, and CUPRAC assay), DPP-IV enzyme inhibitory activity, and a-glucosidase enzyme inhibitory activity of peptides released from in vitro gastrointestinal digested casein and the whey proteins of camel and donkey milk were evaluated. While the highest antioxidant activity was determined to be in the digested camel casein fraction using the ABTS and CUPRAC methods, the digested donkey casein fraction was determined to have the highest radical scavenging activity using the DPPH method. The highest DPP-IV inhibitory activity was detected in digested camel and donkey milk casein fractions. Digested whey fractions of camel and donkey milk had a lower DPP-IV inhibitory activity compared to the digested casein fractions. However, digested whey fractions of camel and donkey milk did not show a-glucosidase inhibitory activity, and digested donkey casein fraction showed the highest a-glucosidase inhibitory activity with a 12.5 lg/mL IC50 value. It was concluded that peptides released from digested casein fraction of camel and donkey milk have potent antioxidant and particularly antidiabetic properties. Keywords Camel milk Donkey milk Bioactive peptides DPP-IV inhibitory activity Antioxidant activity a-glucosidase inhibitory activity
& Ecem Akan [email protected] 1
Faculty of Agriculture, Department of Dairy Technology, Aydın Adnan Menderes University, 09970, Koc¸arlı, Aydın, Turkey
Introduction Type II diabetes is the one of the most serious metabolite syndromes affecting many people every year around the world. In 2017, 451 million people worldwide between the ages of 18 and 99 suffered from Type II diabetes, and by the year 2045, it is predicted that this number will rise to 693 million (Cho et al. 2018). Dipeptidyl peptidase-IV (DPP-IV) and a-glucosidase are enzymes inactivating incretins that regulate a normal blood glucose level (Demuth et al. 2005). Inhibition of DPP-IV and a-glucosidase enzymes is the one of the primary methods of controlling Type II diabetes. From in vivo studies, it has been discovered that food proteins have a role in regulating serum glucose levels (Lacroix and Li-Chan 2013). In these studies, animal and marine products and plants were used as food protein resources for the regulation of glucose levels (Me´ric et al. 2014). Bioactive peptides from several protein sources, particularly milk proteins, have many biological properties including antioxidant, antimicrobial, antidiabetic, antihypertensive, anticancer, and antitumor activities (Ayyash et al. 2018). The DPP-IV inhibitory activities of peptides from bovine milk and its products have been examined
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