A Highly Selective and Sensitive Peptide-Based Fluorescent Ratio Sensor for Ag +
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ORIGINAL ARTICLE
A Highly Selective and Sensitive Peptide-Based Fluorescent Ratio Sensor for Ag+ Shuaibing Yu 1 & Zhaolu Wang 1 & Lei Gao 2 & Bo Zhang 1 & Lei Wang 1 & Jinming Kong 3 & Lianzhi Li 1 Received: 13 August 2020 / Accepted: 12 November 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A fluorescence ratio sensor based on dansyl-peptide, Dansyl-Glu-Cys-Glu-Glu-Trp-NH2 (D-P5), was efficiently synthesized by Fmoc solid phase peptide synthesis. The sensor exhibits high selectivity and sensitivity for Ag+ over 16 metal ions in 100 mM sodium perchlorate and 50 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid buffer solution by fluorescence resonance energy transfer. The 1:1 binding stoichiometry of the sensor and Ag+ is measured by fluorescence ratio response and the job’s plot. The dissociation constant of the sensor with Ag+ was calculated to be 6.4 × 10−9 M, which indicates that the sensor has an effective binding affinity for Ag+. In addition, the limit of detection of the sensor for Ag+ was determined to be 80 nM, which also indicates that the sensor has a high sensitivity to Ag+. Result showed that the sensor is an excellent Ag+ sensor under neutral condition. Furthermore, this sensor displays good practicality for Ag+ detection in river water samples without performing tedious sample pretreatment, as well as for silver chloride detection. Keywords Fluorescence ratio sensor . Peptide . Ag+ . Fluorescence resonance energy transfer
Introduction Silver is a very important metal which is widely used in electrical industry, photographic materials and medical applications. Silver itself has good electrical and thermal conductivity as an electronic material, silver halide can be used as a photosensitive material, and silver ion and silver-containing compounds can kill or inhibit bacteria and viruses [1–3]. On the other hand, due to the non-biodegradability of heavy metals and transition metals, it will accumulate in the environment and organisms [4]. Long-term accumulation of these metals can cause a series of hazards to the environment and organisms [5–9]. Silver ion is considered to be harmful substance
* Lianzhi Li [email protected] 1
School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, People’s Republic of China
2
Zhong Yuan Academy of Biological Medicine, Liaocheng People’s Hospital Affiliated to Shandong University, Liaocheng 252000, People’s Republic of China
3
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, People’s Republic of China
and is also a major environmental pollutant. Moreover, it can damage enzymes and combine with amines and various metabolites, posing a threat to environmental safety and human health [10, 11]. Therefore, a fast and sensitive method for detecting Ag+ is needed. Standard method for detection of silver in water, such as inductively coupled plasma-mass spectrometry (ICPMS) [12], is a complicated and time-consuming procedure.
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