Acute exercise in mice transiently remodels the hepatic lipidome in an intensity-dependent manner
- PDF / 1,982,330 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 101 Downloads / 170 Views
(2020) 19:219
RESEARCH
Open Access
Acute exercise in mice transiently remodels the hepatic lipidome in an intensitydependent manner Gregory C. Henderson1* , Valeria Martinez Tenorio1 and Marc A. Tuazon2
Abstract Background: The content of triacylglycerol (TAG) in the liver is known to rapidly increase after a single bout of exercise followed by recovery to sedentary levels. The response of other hepatic lipids, and acyl chain composition of lipid classes, would provide a deeper understanding of the response of hepatic lipid metabolism to acute exercise. Methods: Female mice performed a single bout of continuous exercise (CE), high-intensity interval exercise (HIIE), or no exercise (CON). The total content of various lipids in the liver, and fatty acids within lipid classes, were measured in tissues collected 3 h after exercise (Day 1) and the day following exercise (Day 2). Results: The total concentration of TAG rose on Day 1 after exercise (P < 0.05), with a greater elevation in HIIE than CE (P < 0.05), followed by a decline toward CON levels on Day 2. The total concentration of other measured lipid classes was not significantly altered by exercise. However, n-6 polyunsaturated fatty acid relative abundance in diacylglycerol (DAG) was increased by HIIE (P < 0.05). In CON liver, TAG content was positively correlated with DAG and phosphatidylethanolamine (P < 0.05), while these statistical associations were disrupted in exercised mice on Day 1. Conclusions: The response of lipid metabolism to exercise involves the coordination of metabolism between various tissues, and the lipid metabolism response to acute exercise places a metabolic burden upon the liver. The present findings describe how the liver copes with this metabolic challenge. The flexibility of the TAG pool size in the liver, and other remodeling of the hepatic lipidome, may be fundamental components of the physiological response to intense exercise. Keywords: Lipidomics, High-intensity interval training, Postexercise recovery, Post-exercise, Intrahepatocellular lipid, Triglyceride
Background The lipid that accumulates in the body during positive energy balance is primarily as triacylglycerol (TAG) in adipose tissue. However, TAG and other lipids can accumulate elsewhere as well at lower concentrations, and * Correspondence: [email protected] 1 Department of Nutrition Science, Purdue University, 700 West State Street, West Lafayette, IN 47907, USA Full list of author information is available at the end of the article
this ectopic lipid deposition is common during weight gain and other states of metabolic dysfunction in laboratory rodents and human subjects [1–6]. This lipid accumulation has serious ramifications for metabolism; excess TAG accumulation in liver and muscle typically presents alongside elevated levels of lipotoxic intermediates such as diacylglycerol (DAG) [1–5]. DAG and even perhaps other lipotoxic intermediates in these insulinresponsive tissues can lead to the development of insulin
© The Author(s). 2020 Open Access This article
Data Loading...
