Analysis of DNA methylation in different tissues of Fenneropenaeus chinensis from the wild population and Huanghai No. 1
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Analysis of DNA methylation in different tissues of Fenneropenaeus chinensis from the wild population and Huanghai No. 1 HE Yuying1, 2, DU Ying3, LI Jian1, 2*, LIU Ping1, WANG Qingyin1, LI Zhaoxia4 1 Key Laboratory for Sustainable Utilization for Marine Fisheries, Yellow Sea Fisheries Research Institute, Chinese
Academy of Fishery Sciences, Qingdao 266071, China 2 Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for
Marine Science and Technology, Qingdao 266235, China 3 College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China 4 College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
Received 01 August 2014; accepted 15 December 2014 ©The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2015
Abstract
DNA methylation plays an important role in the regulation of gene expression during biological development and tissue differentiation in eukaryotes. A methylation sensitive amplification polymorphism (MSAP) including digestion, pre-selective amplification and selective amplification was optimized to compare the levels of DNA cytosine methylation at CCGG sites in muscle, gill and hemocyte from the wild populations and the selective breeding of Huanghai No. 1 of Fenneropenaeus chinensis, respectively. Significant differences in cytosine methylation levels among three tissues in two populations were detected. The average DNA methylation ratios in muscle, gill and hemocyte of the wild population were 23.1%, 22.3% and 19.7%, while those were 21.4%, 19.6%, and 18.9% in Huanghai No. 1, respectively. The DNA methylation levels of gill from the two populations were highly significant (P
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