Anatabine ameliorates intestinal inflammation and reduces the production of pro-inflammatory factors in a dextran sulfat
- PDF / 1,883,452 Bytes
- 17 Pages / 595.276 x 790.866 pts Page_size
- 17 Downloads / 168 Views
(2020) 17:29
RESEARCH
Open Access
Anatabine ameliorates intestinal inflammation and reduces the production of pro-inflammatory factors in a dextran sulfate sodium mouse model of colitis Pedro A. Ruiz Castro1*† , Ulrike Kogel1†, Giuseppe Lo Sasso1†, Blaine W. Phillips2*†, Alain Sewer1†, Bjorn Titz1, Llenalia Garcia1, Athanasios Kondylis1, Emmanuel Guedj1, Dariusz Peric1, David Bornand1, Remi Dulize1, Celine Merg1, Maica Corciulo1, Nikolai V. Ivanov1, Manuel C. Peitsch1 and Julia Hoeng1*
Abstract Background: Inflammatory bowel disease (IBD) is the collective term for chronic immune-mediated diseases of unknown, multifactorial etiology, arising from the interplay between genetic and environmental factors and including two main disease manifestations: ulcerative colitis (UC) and Crohn’s disease. In the last few decades, naturally occurring alkaloids have gained interest because of their substantial anti-inflammatory effects in several animal models of disease. Studies on mouse models of IBD have demonstrated the anti-inflammatory action of the main tobacco alkaloid, nicotine. In addition, anatabine, a minor tobacco alkaloid also present in peppers, tomato, and eggplant presents anti-inflammatory properties in vivo and in vitro. In this study, we aimed to evaluate the anti-inflammatory properties of nicotine and anatabine in a dextran sulfate sodium (DSS) mouse model of UC. Results: Oral administration of anatabine, but not nicotine, reduced the clinical symptoms of DSS-induced colitis. The result of gene expression analysis suggested that anatabine had a restorative effect on global DSS-induced gene expression profiles, while nicotine only had limited effects. Accordingly, MAP findings revealed that anatabine reduced the colonic abundance of DSS-associated cytokines and increased IL-10 abundance. Conclusions: Our results support the amelioration of inflammatory effects by anatabine in the DSS mouse model of UC, and suggest that anatabine constitutes a promising therapeutic agent for IBD treatment. Keywords: Plant-derived alkaloid, Mouse model, Nicotine, Colitis
* Correspondence: [email protected]; [email protected]; [email protected] † Pedro A. Ruiz Castro, Ulrike Kogel, Giuseppe Lo Sasso, Blaine W. Phillips and Alain Sewer contributed equally to this work. 1 Philip Morris International R&D, Philip Morris Products S.A, Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland 2 Philip Morris International Research Laboratories Pte Ltd, 50 Science Park Road, The Kendall #02-07, Science Park II, Singapore 117406, Singapore
© The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons lice
Data Loading...
