Arbuscular mycorrhizal symbiosis alters the expression of PHT1 phosphate transporters in roots and nodules of P-starved
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Arbuscular mycorrhizal symbiosis alters the expression of PHT1 phosphate transporters in roots and nodules of Pstarved soybean plants Rafaela Gageti Bulgarelli . Vinicius H. De Oliveira . Sara Adria´n Lo´pez de Andrade
Received: 28 April 2020 / Accepted: 8 August 2020 / Published online: 14 August 2020 Ó Brazilian Society of Plant Physiology 2020
Abstract Roots acquire phosphorus (P) as orthophosphate (Pi) through phosphate transporters of the PHT1 family with different affinities to Pi, a process significantly influenced by arbuscular mycorrhizal (AM) symbiosis. However, scarce P availability may constrain nodulation and performance in legumes. Soybean (Glycine max) is an ideal model to study tripartite symbiosis between roots, AM fungi and rhizobia. To evaluate AM influence on GmPHT1 expression in roots and nodules, Bradyrhizobium elkanii-inoculated soybean plants were exposed to low Pi concentration (50 lM) via nutrient solution and inoculated (? AM) or not (- AM) with the AM fungus Glomus macrocarpum. Control treatment consisted of non-inoculated plants grown under sufficient P conditions (500 lM; - AM ? P). Plants were collected at the flowering and grain filling stages. Under P-starvation, mycorrhizal plants showed low intraradical colonization and did not differ in terms of
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s40626-020-00185-8) contains supplementary material, which is available to authorized users. R. G. Bulgarelli V. H. De Oliveira S. A. L. de Andrade (&) Laboratory of Plant Molecular Physiology (LaFiMP), Department of Plant Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP 13083- 970, Brazil e-mail: [email protected]
biomass, nodulation and P content from the nonmycorrhizal plants, indicating strong P-limitation and no AM-related growth promotion. However, the expression profile of PHT1 transporters in roots and nodules was effectively altered by mycorrhization. P-starvation induced the expression of several GmPHT1 genes in roots and nodules, while AM symbiosis repressed GmPHT1;6, 7 and 10 in roots and GmPHT1;3, 5, 7, 8, and 10 in nodules. Therefore, even under low levels of root colonization, AM symbiosis significantly modulated the pattern of PHT1 expression under P-starvation. GmPHT1 expression profile suggests different pathways of Pi acquisition in mycorrhizal and non-mycorrhizal plants during P-starvation, however, the low mycorrhizal colonization was not able to deliver adequate P nutrition to the plant. Keywords PHT1 transporters Inorganic phosphate Nodulation Tripartite symbiosis
1 Introduction Nitrogen (N) and phosphorus (P) are the nutrients that most limit plant growth and crop production around the world (Elser et al. 2007). Legumes are able to form associations with both N2-fixing bacteria and with arbuscular mycorrhizal (AM) fungi in a tripartite
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symbiosis with roots (Vance et al. 2003). The tripartite interaction may benefit the plant through N2-fixation and improvement of P up
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