Assessment of yeasts in tropical peat swamp forests in Thailand
- PDF / 2,469,937 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 68 Downloads / 241 Views
ORIGINAL ARTICLE
Assessment of yeasts in tropical peat swamp forests in Thailand Rujikan Nasanit 1 & Napakhwan Imklin 1 & Savitree Limtong 2,3 Received: 8 July 2020 / Revised: 25 October 2020 / Accepted: 1 November 2020 # German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Despite the abundance of yeasts in peatland, only a few studies on peatland yeasts have been reported and, further, most of those studies were carried out in boreal regions. In response, this study assesses yeast communities in tropical peat swamp forests (PSFs). Peat soils or soils from a primary or natural PSF were collected in Thailand at Khanthuli PSF (PKT), while those from secondary or disturbed PSFs were collected from Khanthuli (SKT) and Khuankreng PSFs (SKK). Clone libraries of each sample were separately constructed using PCR products of the D1/D2 domains of the large subunit ribosomal DNA. Most of the yeast operational taxonomic units (OTUs) detected in Khanthuli PSF, both PKT and SKT, belonged to the phylum Ascomycota. Meanwhile, most of the yeast OTUs in SKK belonged to Basidiomycota. Each PSF area exhibited a specific yeast community. Only a few OTUs were shared between the different areas. The dominant yeast species in PKT and SKT was Galactomyces candidus, whereas in SKK, it was Solicoccozyma keelungensis. Saccharomyces cerevisiae was the only common species in each area. In addition, many more potential new yeast taxa are still undiscovered in the peatlands. The canonical correspondence analysis (CCA) showed that the abundance of G. candidus was related to sites with higher nitrogen concentrations and higher pH, while the abundance of So. Keelungensis was related to a combination of pH and electrical conductivity rather than total potassium contents. These results suggest that yeast communities in primary and secondary PSFs were different, which may be due to both biotic and abiotic factors. Keywords D1/D2 domain . PCR . Peat swamp forest . Soil yeast . Yeast diversity
Introduction Regarded as a unique type of ecosystem, peatlands cover an estimated area of 400 million hectares (ha) worldwide. Most peatlands (approximately 350 million ha) cover boreal and Section Editor: Roland Kirschner * Savitree Limtong [email protected] Rujikan Nasanit [email protected] Napakhwan Imklin [email protected] 1
Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Sanamchandra Palace Campus Nakhon Pathom 73000 Thailand
2
Department of Microbiology, Faculty of Science, Kasetsart University, Jatujak Bangkok 10900 Thailand
3
Academy of Science, The Royal Society of Thailand, Bangkok 10300 Thailand
sub-Arctic zones where climates are characterized by high precipitation and low temperatures. Tropical peatlands are typically found in tropical lowlands surrounded by rainforests on well-drained soils or by saltwater mangrove forests near coastlines (Page` et al. 2006). Waterlogged soil allows organic matter to slowly decompose, which results in the accumula
Data Loading...
