Biochemical coupling strategy promotes saccharification of bamboo leaves biomass via xylanase and heteropolyacids
- PDF / 2,206,622 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 41 Downloads / 147 Views
ORIGINAL ARTICLE
Biochemical coupling strategy promotes saccharification of bamboo leaves biomass via xylanase and heteropolyacids Zhuqian Xiao 1,2
&
Qiang Zhang 1 & Xiaolei Wang 1 & Qing Ge 1 & Jianwei Mao 1,2 & Qinqin Yang 1 & Jianbing Ji 2
Received: 4 April 2019 / Revised: 26 August 2019 / Accepted: 13 September 2019 # Springer-Verlag GmbH Germany, part of Springer Nature 2019
Abstract This study describes a promising strategy coupled heteropolyacids and enzyme to saccharification of raw bamboo leaves biomass. High yield of saccharides is scalable using this coupling technology. The phosphotungstic acid (PTA) and xylanase are adopted in this catalytic system and performed to produce xylose and polysaccharides with potential high activity. 317.7 mg/g of total monosaccharides and 170.0 mg/g polysaccharides (48.7% yield of total saccharides) are achieved by H3PW12O40 under 150 °C for 2.0 h successively coupled 200 μL xylanase digesting for 7.0 h. The coupling strategy of heteropolyacids and enzyme could promote respective advantages because Brønsted acid sites in PTA could regularly degrade the hemicellulose and cellulose to generate smaller molecules and successively expose more reactive sites to enzymes. DPPH and ABTS radicals scavenging activities and reducing power investigations further prove the polysaccharides exhibit strong antioxidant activity compared to the concentrated vitamin C. This method may aid in fast production of monosaccharides and bio-active polysaccharides for both of healthy medicine and food industry. Keywords Bamboo leaves . Coupling catalysis . Monosaccharides . Polysaccharides . Biomass
1 Introduction Biomass, lignocellulose included, originating from the photosynthesis of water and carbon dioxide catalyzed by solar light is one of the renewable resource in nature. The lignocellulose is the main constitute of cell wall abundant in plants such as trees, bush and some graminaceous plants. The cellulose, hemicellulose and lignin linked by β-1,4-glycosidic bonds and β-O-4 ether bonds are the three main components to fabricate robust structure of lignocellulose [1, 2]. Due to its their
* Zhuqian Xiao [email protected] * Jianwei Mao [email protected] 1
Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, Zhejiang University of Science and Technology, Hangzhou 310023, People’s Republic of China
2
College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, People’s Republic of China
carbon neutrality and renewability, the refinery of lignocellulose to platform chemicals, mainly including aldehydes, organic acids, polyols, phenols, saccharides and even amino acids, has been regarded as an alternative strategy to reduce use of fossil resources [3–10, 51]. Bamboo is one of tropical graminaceous plants and some of them are medical and edible, and about 75 genera and 1250 species of bamboo grow worldwide, especially in China. For another hand, bamboo stem contains 40–48% cellulose, 25–30% hemicellulose and
Data Loading...
