Biodiversity of meatborne Listeria spp. in Himachal Pradesh and their interaction with indigenous probiotics
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Biodiversity of meatborne Listeria spp. in Himachal Pradesh and their interaction with indigenous probiotics Aakriti Sharma1 • S. S. Kanwar1 • Sidharath Dev Thakur2
Revised: 2 October 2020 / Accepted: 8 October 2020 Ó Association of Food Scientists & Technologists (India) 2020
Abstract This study determined the anti-listerial activity of indigenous probiotics from traditional fermented foods of Western Himalaya against meat borne Listera monocytogens isolates from Himachal Pradesh. One hundred samples of meat and meat products like chicken (n = 25), chevon (goat meat, n = 20), fish (n = 20) and pork (n = 30) were collected and were analyzed for the presence of Listeria spp. by recommended culture and biochemical methods. L. monocytogens isolates were confirmed by PCR targeting the virulence gene hlyA (haemolysin A) and by16S rRNA sequencing. Anti-listerial activity of probiotic bacteria isolated from indigenous fermented foods of Himachal Pradesh was determined by well diffusion method using Lactobacillus rhamnosus GG (ATCC 53103) as the reference strain. Five percent of tested samples were found positive for L. monocytogens with incidence of 8.0% in chicken (2/25), 10.0% in fish (2/20) and 4.0% in chevon meat (1/25). None of the tested pork samples were found contaminated with L. monocytogenes. Among 11 indigenous probiotics used in this study, highest antagonistic activity was exhibited by Lactobacillus plantarum (ADF
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13197-020-04854-z) contains supplementary material, which is available to authorized users. & Sidharath Dev Thakur [email protected] 1
Department of Microbiology, College of Basic Sciences, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh 176062, India
2
Department of Veterinary Public Health and Epidemiology, Dr. GC Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh 176062, India
10) and Enterococcus faecium (ADF1) which was equivalent to the reference strain. Keywords Listeria monocytogenes Lactobacillus Enterococcus hlyA
Introduction Human listeriosis is an important food borne illness with worldwide distribution. It is caused by Listeria monocytogenes, a Gram positive, facultative anaerobic, rod shaped, motile and non-spore forming bacterium (Orsi and Wiedmann 2016). Most infected individuals experience mild to severe gastroenteritis, but listeriosis can result in septicemia, fetal infections, abortions, stillbirths, prenatal infections, swelling of the salivary glands and the nuchal lymph nodes, liver abscesses, endocarditis, arthritis and meningitis and meningoencephalitis in pregnant women, children and immune-compromised and elderly individuals (Radoshevich and Cossart 2018; Bauerfeind et al. 2016). Despite its rare incidences, listeriosis remains a great public health concern and can cause 20–30% mortality among infected individuals (de Noordhout et al. 2014).
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