C. elegans Methods and Applications
The aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab. Each protocol in this volume is presented as a standalone chapter, specifically geared towards addressing practical needs without presum
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David Biron Gal Haspel Editors
C.elegans Methods and Applications Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
C. elegans Methods and Applications Second Edition
Edited by
David Biron Department of Physics, The James Franck Institution, The Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA
Gal Haspel Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, NJ, USA
Editors David Biron Department of Physics The James Franck Institution The Institute for Biophysical Dynamics The University of Chicago Chicago, IL, USA
Gal Haspel Federated Department of Biological Sciences New Jersey Institute of Technology and Rutgers University Newark, NJ, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2841-5 ISBN 978-1-4939-2842-2 (eBook) DOI 10.1007/978-1-4939-2842-2 Library of Congress Control Number: 2015945342 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Tractable models such as the nematode Caenorhabditis elegans provide profound insight into a wide range of biological phenomena. It is the objective of any scientific effort to understand a natural phenomenon. Considerable effort and ingenuity are dedicated towards developing novel methods and approaches that will enable breakthroughs and progress. C. elegans has a short life cycle—it matures during three days at room temperature— and is easy to cultivate. Under standard laboratory conditions, hermaphroditic nematodes self-fertilize but mati
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