Changes in cell wall structure and protein set in Candida maltosa grown on hexadecane
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ORIGINAL ARTICLE
Changes in cell wall structure and protein set in Candida maltosa grown on hexadecane Anton Zvonarev 1 & Vasilina Farofonova 1 & Ekaterina Kulakovskaya 1 & Tatiana Kulakovskaya 1 Svyatoslav Sokolov 2 & Vladimir Dmitriev 1
&
Andrey Machulin 1 &
Received: 24 April 2020 / Accepted: 18 November 2020 # Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2020
Abstract The yeast Candida maltosa is a model organism for studying adaptive changes in the structure and function of the cell wall when consuming water-insoluble nutrient sources. The cells of C. maltosa that utilize hydrocarbons contain supramolecular structures, so-called “canals” in the cell wall. Differences in protein profiles of culture liquids and cell wall extracts of C. maltosa grown on glucose and hexadecane were analyzed. Three proteins specific of cells grown on hexadecane were revealed using mass spectrometry: glycosyl hydrolase EPD2 in the culture liquid; a protein belonging to the cytochrome C family in the 0.5 mol/L NaCl extract; and PPIA_CANAL protein known as chaperone, in the 0.1% SDS extract. The possible role of these proteins in cell wall structures responsible for adaptation to hexadecane utilization is discussed.
Introduction The yeast cell wall provides a direct contact with the environment and plays an important role in the adaptation of cells to various carbon sources, their development, aging, stress response, morphogenesis, and adhesion (Sanz et al. 2017; Molon et al. 2018; Willaert 2018; Davì et al. 2019). The role of cell wall proteins in these processes is being intensively studied (Sanz et al. 2017; Sabirzyanov et al. 2018; Liu et al. 2018; Huang et al. 2018). The cell wall of pathogenic Candida species plays a key role in the fungi-host interaction, biofilm formation, and drug resistance (Dižová et al. 2018; Suchodolski et al. 2020; Yeh et al. 2020; Garcia-Rubio et al. 2020). The yeast Candida maltosa is a model organism for studying adaptive changes in the structure and function of the cell wall when consuming water-insoluble nutrient sources. In the cell wall of C. maltosa, which consumes hexadecane, original
* Tatiana Kulakovskaya [email protected] 1
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, prosp. Nauki 5, Pushchino, Moscow Region 142290, Russia
2
Belozersky Research Institute of Physico-Chemical Biology, MSU, Leninskiye Gory, Moscow 119899, Russia
supramolecular structures, the so-called “canals,” were found, and specific polysaccharides and proteins that carry out oxidative reactions were associated with these structures (Dmitriev et al. 2011; Dmitriev et al. 2016; Zvonarev et al. 2017). The metabolism of hydrocarbons and their derivatives in yeasts of different taxonomic groups is well studied (Fukuda 2013; Finogenova et al. 2005; Beier et al. 2014). The products of many genes are involved in the assimilation of n-alkanes in yeasts (Mori et al. 2013). The expre
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