Characteristics of the spike and ORF3 genes of porcine epidemic diarrhea virus in Henan and Shanxi provinces of China
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Characteristics of the spike and ORF3 genes of porcine epidemic diarrhea virus in Henan and Shanxi provinces of China Jian‑tao Cui1 · Han Qiao2 · Cheng‑yao Hou1 · Hui‑Hua Zheng1 · Xin‑Sheng Li1 · Lan‑lan Zheng1 · Hong‑Ying Chen1 Received: 13 March 2020 / Accepted: 22 June 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract To investigate the epidemic characteristics of porcine epidemic diarrhea virus (PEDV), 135 clinical samples (including intestinal tissues and feces) were collected from diseased piglets during outbreaks of diarrhea from 2015 to 2019 on farms in Henan and Shanxi provinces of China where swine had been immunized with attenuated PEDV (CV777). A total of 86 clinical samples (86/135, 63.7%) were positive for PEDV by RT-PCR, and subsequently, the complete spike (S) and ORF3 genes of 32 PEDV samples were sequenced. Phylogenetic analysis showed that the 32 PEDV strains obtained in this study belonged to group 2 (pandemic variant strains) and had a close relationship to 17 Chinese strains after 2010, two South Korean strains (KNU-1305 and KNU-1807), three American strains (PC22A-P140.BI, USA/Colorado/2013, and USA/ OK10240-6/2017) and a Mexican strain (PEDV/MEX/QRO/02/2017), but differed genetically from a South Korean strain (SM98), a European strain (Br1/87), a Chinese strain (LZC), and a vaccine strain (CV777). G2-a subgroup strains were the dominant pandemic variant strains circulating in Henan and Shanxi provinces of China. Furthermore, a cross-recombination event was identified in the S region of the SX/TY2/2017 strain, and the putative parental strains were the epidemic strains CH/GDGZ/2012 and CH/YZ1/2015, identified in China in 2012 and 2015, respectively. These results provide further information about PEDV evolution, which could improve our understanding of the circulation of PEDV in Henan and Shanxi provinces. This information will also be helpful for developing new strategies for prevention and control of variant strains.
Introduction Porcine epidemic diarrhea virus (PEDV) has been recognized in veterinary medicine since the early 1970s in Europe and has subsequently been detected in many other swinebreeding areas throughout the world [27]. The virus is the Handling Editor: Sheela Ramamoorthy. Jian-tao Cui, Han Qiao and Cheng-yao Hou contributed equally to this work. * Lan‑lan Zheng [email protected] * Hong‑Ying Chen [email protected] 1
Zhengzhou Key Laboratory for Pig Disease Prevention and Control, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Nongye Road 63#, Zhengdong New District, Longzi Lake #15, Zhengzhou 450046, Henan, People’s Republic of China
College of Life Science, South China Agricultural University, Guangzhou 510642, Guangdong, People’s Republic of China
2
causative agent of porcine epidemic diarrhea (PED), an acute and highly contagious enteric disease characterized by vomiting, watery diarrhea, dehydration, and high mortality in suckling piglets [8, 24]. Swine is the only host capable of a pro
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