Chemokines during anaphylaxis: the importance of CCL2 and CCL2-dependent chemotactic activity for basophils
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Clinical and Translational Allergy Open Access
RESEARCH
Chemokines during anaphylaxis: the importance of CCL2 and CCL2‑dependent chemotactic activity for basophils Romana Vantur1, Marusa Rihar1, Ana Koren1, Matija Rijavec1,2, Peter Kopac1,3, Urska Bidovec‑Stojkovic1, Renato Erzen1,3 and Peter Korosec1*
Abstract Background: The role of chemokines in anaphylaxis is unclear. Methods: We prospectively recruited 49 patients presenting to the emergency department with an acute episode of anaphylaxis and 28 healthy subjects. We measured serum levels of the chemokines CCL2, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL21, CCL22, CCL24, and CCL26, tryptase, the absolute number of circulating basophils, monocytes, lymphocytes, and PMNs, and whole blood FCER1A, CPA3 and HDC gene expression at two time points: during the ana‑ phylactic episode and in convalescent samples collected approximately 3 months later. We then investigated the in vitro chemotactic activity of chemokines induced during anaphylaxis for the in vitro migration of the corresponding cells. Results: Only CCL2 chemokine levels were significantly increased in anaphylaxis samples (median 514 pg/ml) compared to convalescent samples (284 pg/ml, P
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