Comparative proteomic analysis provides new insight into differential transmission of two begomoviruses by a whitefly
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RESEARCH
Open Access
Comparative proteomic analysis provides new insight into differential transmission of two begomoviruses by a whitefly Jing Zhao†, Yao Chi†, Xin-Jia Zhang, Teng Lei, Xiao-Wei Wang and Shu-Sheng Liu*
Abstract Background: Viruses in the genus Begomovirus (Family Geminiviridae) include many important economic plant viruses transmitted by whiteflies of the Bemisia tabaci species complex. In general, different begomoviruses may be acquired and transmitted by the same whitefly species with different efficiencies. For example, the species Mediterranean (MED) in this whitefly species complex transmits tomato yellow leaf curl virus (TYLCV) at a higher efficiency than papaya leaf curl China virus (PaLCuCNV). However, the proteomic responses of whitefly to the infection of different begomoviruses remain largely unknown. Methods: We used iTRAQ-based proteomics coupled with RT-qPCR to investigate and compare responses of the MED whitefly to the infection of TYLCV and PaLCuCNV. Results: Totally, 259, 395 and 74 differently expressed proteins (DEPs) were identified in the comparisons of TYLCVinfected vs. un-infected, PaLCuCNV-infected vs. un-infected, and TYLCV-infected vs. PaLCuCNV-infected whiteflies, respectively. These proteins appear associated with catabolic process, metabolic process, transport, defense response, cell cycle, and receptor. The comparisons of TYLCV-infected vs. un-infected and PaLCuCNV-infected vs. un-infected shared some similar DEPs, indicating possible involvement of laminin subunit alpha, dystroglycan, integrin alpha-PS2 and cuticle proteins in viral transport as well as the role of putative defense proteins 3 and PITH in anti-viral response. However, 20S proteasome subunits associated with regulation of virus degradation and accumulation were up-regulated in PaLCuCNVinfected but not in TYLCV-infected whiteflies, which may be related to the constraints of PaLCuCNV accumulation in MED. Conclusions: These findings provide valuable clues for unravelling the roles of some whitefly proteins in begomovirus transmission. Keywords: Whitefly, Begomovirus, Virus transmission, iTRAQ-based proteomics
Background Plant diseases caused by begomoviruses have been major constraints to the production of many economic crops such as tomato and cotton [1]. Begomoviruses are a group of single-stranded circular DNA viruses with twinned particles, and their genetic structure is bipartite or monopartite [2]. So far, more than 388 species have been described in the genus Begomovirus (Family Geminiviridae) [3], which in general are transmitted by whiteflies in the Bemisia tabaci species complex in a persistent, circulative manner * Correspondence: [email protected] † Jing Zhao and Yao Chi contributed equally to this work. Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, People’s Republic of China
[4]. For begomoviruses, once orally acquired by whitefly, they follow the path of head-midgut-haemolym
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